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Urethral striated muscle and extracellular matrix morphological characteristics among mildly diabetic pregnant rats: translational approach

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Autor(es):
Piculo, Fernanda [1] ; Marini, Gabriela [1] ; Pascon Barbosa, Angelica Mercia [1] ; Damasceno, Debora Cristina [1] ; Michelin Matheus, Selma Maria [2] ; Felisbino, Sergio Luis [3] ; Daneshgari, Firouz [4] ; Cunha Rudge, Marilza Vieira [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, Sao Paulo - Brazil
[2] Univ Estadual Paulista UNESP, Dept Anat, Inst Biosci, Sao Paulo - Brazil
[3] Univ Estadual Paulista UNESP, Dept Morphol, Inst Biosci, Sao Paulo - Brazil
[4] Case Western Reserve Univ, Urol Inst Univ Hosp, Case Med Ctr, Dept Urol, Cleveland, OH 44106 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL UROGYNECOLOGY JOURNAL; v. 25, n. 3, p. 403-415, MAR 2014.
Citações Web of Science: 8
Resumo

Diabetes mellitus (DM) during pregnancy is associated with high levels of urinary incontinence (UI) and pelvic floor muscle dysfunction. Mild DM can lead to changes in urethral striated muscle and extracellular matrix (ECM) in pregnant rats considering both structures as an entire system responsible for urinary continence. Ninety-two female Wistar rats were distributed in four experimental groups: virgin, pregnant, diabetic, and diabetic pregnant. In adult life, parental nondiabetic female rats were mated with nondiabetic male rats to obtain newborns. At the first day of birth, newborns received citrate buffer (nondiabetic group) or streptozotocin 100 mg/kg body weight, subcutaneous route (mild DM group). At day 21 of the pregnancy, the rats were lethally anesthetized and the urethra and vagina were extracted as a unit. Urethral and vaginal sections were cut and analyzed by: (a) cytochemical staining for ECM and muscle structural components, (b) immunohistochemistry to identify fast- and slow-muscle fibers, and (c) transmission electron microscopy for ultrastructural analysis of urethral striated muscle. In comparison with the three control groups, variations in the urethral striated muscle and ECM from diabetic pregnant rats were observed including thinning, atrophy, fibrosis, increased area of blood vessels, mitochondria accumulation, increased lipid droplets, glycogen granules associated with colocalization of fast and slow fibers, and a steady decrease in the proportion of fast to slow fibers. Mild DM and pregnancy can lead to a time-dependent disorder and tissue remodeling in which the urethral striated muscle and ECM has a fundamental function. (AU)

Processo FAPESP: 12/00099-4 - Análise estrutural da matriz extracelular e do músculo estriado uretral em ratas prenhes diabéticas
Beneficiário:Fernanda Piculo
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
Processo FAPESP: 10/13303-3 - Efeito do diabete moderado na matriz extracelular e no músculo estriado uretral em ratas prenhes
Beneficiário:Fernanda Piculo
Linha de fomento: Bolsas no Brasil - Mestrado