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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Photodynamic therapy in the cattle protozoan Tritrichomonas foetus cultivated on superhydrophilic carbon nanotube

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Autor(es):
Machado, Susane Moreira [1] ; Pacheco-Soares, Cristina [1, 2] ; Marciano, Fernanda Roberta [3] ; Lobo, Anderson Oliveira [3] ; da Silva, Newton Soares [1, 2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Vale Paraiba UNIVAP, Dev & Res Inst IP&D, Lab Tissue & Cell Biol, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[2] Univ Vale Paraiba UNIVAP, Dev & Res Inst IP&D, Lab Dynam Cellular Compartments, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[3] Univ Vale Paraiba UNIVAP, Dev & Res Inst IP&D, Lab Biomed Nanotechnol, BR-12244000 Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 36, p. 180-186, MAR 1 2014.
Citações Web of Science: 5
Resumo

Superhydrophilic vertically aligned carbon nanotubes (VACNT-O-2) were used for the first time as scaffolds for photodynamic therapy (PDT) to induce inhibition of cell division in eukaryotic cells. VACNT-O-2 scaffolds were produced on Ti substrates using plasma enhanced chemical vapor deposition technique and functionalized by oxygen plasma. Scanning electron microscopy (SEM) analysis was performed to characterize the surface changes of the protozoan and interaction with VACNT-O-2. Characterization of lipid and total protein expression was performed with protozoa that were or not treated with PDT. Quantification of protein was conducted using Qubit fluorometer and separated on a polyacrylamide gel. SEM analysis showed the release of lipid vesicles by protozoa after the PDT. These vesicles were characterized by the PKH26 fluorescent probe. The results demonstrated a greater amount of protein released after PDT than in the control. When analyzing the protein material in polyacrylamide gel, a significant protein expression of approximately 65 kDa was found. A model identified the programmed death of Tritrichomonas foetus after the PDT was also proposed. (C) 2013 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
Beneficiário:Fernanda Roberta Marciano
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
Beneficiário:Anderson de Oliveira Lobo
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 08/06654-4 - Terapia fotodinâmica e ativação de morte celular no protozoário parasita do gado bovino Tritrichomonas foetus
Beneficiário:Newton Soares da Silva
Linha de fomento: Auxílio à Pesquisa - Regular