Analysis of HIV-1 Protease Gene Reveals Frequent Multiple Infections Followed by Recombination among Drug Treated Individuals Living in Sao Paulo and Santos, Brazil

Autor(es)

De Morais Nunes, Edsel Renata ^[1] ; Zukurov, Jean Paulo ^[1] ; Maricato, Juliana Terzi ^[2] ; Araripe Sucupira, Maria Cecilia ^[1] ; Diaz, Ricardo Sobhie ^[1] ; Ramos Janini, Luiz Mario ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Med, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 9, n. 1 JAN 3 2014.
• Citações Web of Science: 1

Resumo

The present study investigated the prevalence of HIV-1 multiple infections in a population composed by 47 patients under HAART failure and enrolled at the National DST/AIDS, Program, Ministry of Health, Brazil. Detection of multiple infections was done using a previously published RFLP assay for the HIV-1 protease gene, which is able of distinguishing between infections caused by a single or multiple HIV-1 subtypes. Samples with multiple infections were cloned, and sequence data submitted to phylogenetic analysis. We were able to identify 17 HIV-1 multiple infections out of 47 samples. Multiple infections were mostly composed by a mixture of recombinant viruses (94%), with only one case in which protease gene pure subtypes B and F were recovered. This is the first study that reports the prevalence of multiple infections and intersubtype recombinants in a population undergoing HAART in Brazil. Based on the data there was a steep increase of multiple infections after the introduction of the combined antiretroviral therapy in Brazil. Cases of multiple infections may be associated with HIV-1 genetic diversity through recombination allowing for the generation of viruses showing a combination of resistance mutations. (AU)

• Processo FAPESP: 09/11216-9 - Estudo da Prevalência das Infecções Duplas do HIV-1 em indivíduos em falha virológica submetidos ao Tratamento Antiretroviral Intensivo.
• Beneficiário: Edsel Renata de Morais Nunes
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/10915-0 - Estudo da prevalência das infecções duplas do HIV-1 em indivíduos em falha virológica submetidos ao tratamento antiretroviral intensivo
• Beneficiário: Luiz Mário Ramos Janini
• Modalidade de apoio: Auxílio à Pesquisa - Regular