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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Characterization and Quantification of Compounds in the Hydroalcoholic Extract of the Leaves from Terminalia catappa Linn. (Combretaceae) and Their Mutagenic Activity

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Autor(es):
Mininel, Francisco Jose [1] ; Leonardo Junior, Carlos Sergio [1] ; Espanha, Livia Greghi [2] ; Resende, Flavia Aparecida [2] ; Varanda, Eliana Aparecida [2] ; Fujimura Leite, Clarice Queico [2] ; Vilegas, Wagner [3] ; dos Santos, Lourdes Campaner [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Inst Chem, Dept Organ Chem, BR-14800900 Araraquara, SP - Brazil
[2] Sao Paulo State Univ UNESP, Fac Pharmaceut Sci, Dept Biol Sci, BR-14801902 Araraquara, SP - Brazil
[3] Sao Paulo State Univ UNESP, BR-11350000 Sao Vicente, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Evidence-based Complementary and Alternative Medicine; 2014.
Citações Web of Science: 4
Resumo

Terminalia is a genus of Combretaceous plants widely distributed in tropical and subtropical regions. Thus, the aim of this study was to quantify the majority compounds of the hydroalcoholic extract (7 : 3, v/v) of the leaves from T. catappa by HPLC-PDA, chemically characterize by hyphenated techniques (HPLC-ESI-IT-MSn) and NMR, and evaluate its mutagenic activity by the Salmonella/microsome assay on S. typhimurium strains TA98, TA97a, TA100, and TA102. The quantification of analytes was performed using an external calibration standard. Punicalagin is themost abundant polyphenol found in the leaves. The presence of this compound as a mixture of anomers was confirmed using HPLC-PDA and H-1 and C-13 NMR. Mutagenic activity was observed in strains TA100 and TA97a. As the extract is a complex mixture of punicalagin, its derivatives, and several other compounds, the observed mutagenicity may be explained in part by possible synergistic interaction between the compounds present in the extract. These studies show that mutagenic activity of T. catappa in the Ames test can only be observed when measured at high concentrations. However, considering the mutagenic effects observed for T. catappa, this plant should be used cautiously for medicinal purposes. (AU)

Processo FAPESP: 09/52237-9 - Fitoterápicos padronizados como alvo para o tratamento de doenças crônicas
Beneficiário:Wagner Vilegas
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático