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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Deregulation of Annexin-A1 and Galectin-1 Expression in Precancerous Gastric Lesions: Intestinal Metaplasia and Gastric Ulcer

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Autor(es):
Teixeira Rossi, Ana Flavia [1] ; Duarte, Marcia Cristina [1] ; Poltronieri, Ayla Blanco [1] ; Valsechi, Marina Curado [1] ; Jorge, Yvana Cristina [1] ; Neto, Dalisio de-Santi [2, 3] ; Rahal, Paula [1] ; Oliani, Sonia Maria [1] ; Silva, Ana Elizabete [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Hosp Base, Dept Legal Med, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil
[3] Hosp Base, Pathol Serv, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Mediators of Inflammation; 2014.
Citações Web of Science: 12
Resumo

Objective. Annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) are mediators that play an important role in the inflammatory response and are also associated with carcinogenesis. We investigated mRNA and protein expression in precancerous gastric lesions that participate in the progression cascade to gastric cancer, such as intestinal metaplasia (IM) and gastric ulcer (GU). Methods. Quantitative real-time PCR (qPCR) and immunohistochemical techniques were used to analyze the relative quantification levels (RQ) of ANXA1 and LGALS1 mRNA and protein expression, respectively. Results. Increased relative expression levels of ANXA1 were found in 100% of cases, both in IM (mean RQ = 6.22 +/- 0.06) and in GU (mean RQ = 6.69 +/- 0.10). However, the LGALS1 presented basal expression in both groups (IM: mean RQ = 0.35 +/- 0.07; GU: mean RQ = 0.69 +/- 0.09). Immunohistochemistry revealed significant positive staining for both the AnxA1 and Gal-1 proteins in the epithelial nucleus and cytoplasm as well as in the stroma of the IM and GU groups (P < 0.05) but absence or low immunorectivity in normal mucosa. Conclusion. Our results bring an important contribution by evidencing that both the AnxA1 and Gal-1 anti-inflammatory proteins are deregulated in precancerous gastric lesions, suggesting their involvement in the early stages of gastric carcinogenesis, possibly due to an inflammatory process in the gastric mucosa. (AU)

Processo FAPESP: 12/15036-8 - Avaliação da expressão gênica e protéica em processos inflamatórios e neoplasias do trato digestório: estômago e colorretal
Beneficiário:Ana Elizabete Silva
Linha de fomento: Auxílio à Pesquisa - Regular