Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2O2 Induced Toxicity on Dental Pulp Cells

Autor(es)

Vargas, Fernanda da Silveira ^[1] ; Soares, Diana Gabriela ^[1] ; Dias Ribeiro, Ana Paula ^[2] ; Hebling, Josimeri ^[3] ; De Souza Costa, Carlos Alberto ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Oral Rehabil Program, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Brasilia, Sch Dent, Dept Operat Dent, BR-70910900 Brasilia, DF - Brazil

^[3] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 Araraquara, SP - Brazil

^[4] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMED RESEARCH INTERNATIONAL; 2014.
• Citações Web of Science: 9

Resumo

The aim of this study was to evaluate the protective effects of different concentrations of vitamin E alpha-tocopherol (alpha-T) isomer against the toxicity of hydrogen peroxide (H2O2) on dental pulp cells. The cells (MDPC-23) were seeded in 96-well plates for 72 hours, followed by treatment with 1, 3, 5, or 10 mM alpha-T for 60 minutes. They were then exposed or not to H2O2 for 30 minutes. In positive and negative control groups, the cells were exposed to culture medium with or without H2O2 (0.018%), respectively. Cell viability was evaluated by MTT assay (Kruskal-Wallis and Mann-Whitney tests; alpha = 5%). Significant reduction of cell viability (58.5%) was observed in positive control compared with the negative control. Cells pretreated with alpha-T at 1, 3, 5, and 10 mM concentrations and exposed to H2O2 had their viability decreased by 43%, 32%, 25%, and 27.5%, respectively. These values were significantly lower than those observed in the positive control, thereby showing a protective effect of alpha-T against the H2O2 toxicity. Overall, the vitamin E alpha-T isomer protected the immortalized MDPC-23 pulp cells against the toxic effects of H2O2. The most effective cell protection was provided by 5 and 10 mM concentrations of alpha-T. (AU)

• Processo FAPESP: 11/12938-8 - Novos parâmetros para o clareamento dental: avaliação da eficácia, citotoxicidade e efeitos moleculares
• Beneficiário: Carlos Alberto de Souza Costa
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/15366-5 - Efeito do tratamento de células odontoblastóides com agentes antioxidantes (alfa-tocoferol e Trolox) sobre a atividade citopática trans-amelodentinária de um gel com 35% de peróxido de hidrogênio
• Beneficiário: Fernanda da Silveira Vargas
• Linha de fomento: Bolsas no Brasil - Mestrado