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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Complex interaction between anandamide and the nitrergic system in the dorsolateral periaqueductal gray to modulate anxiety-like behavior in rats

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Autor(es):
Lisboa, S. F. [1, 2] ; Magesto, A. C. [2, 1] ; Aguiar, J. C. [1, 2] ; Resstel, L. B. M. [1, 2] ; Guimaraes, F. S. [2, 1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ctr Interdisciplinary Res Appl Neurosci NAPNA, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Pharmacol, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Neuropharmacology; v. 75, n. SI, p. 86-94, DEC 2013.
Citações Web of Science: 10
Resumo

Stimulation of cannabinoid CBI receptors or inhibition of nitric oxide synthase (NOS) in the dorsolateral periaqueductal gray (dlPAG) decreases anxiety-like behavior. Moreover, activation of CBI receptors attenuates flight responses induced by nitric oxide (NO) donors in the dlPAG, suggesting that endocannabinoids and NO could interact to control defensive responses such as anxiety-like behavior. To test this hypothesis male Wistar rats received intra-dlPAG microinjections of anandamide (AEA) or NO inhibitors and were tested in the elevated plus maze (EPM). Combined administration of low and ineffective doses of AEA and the NO scavenger (c-Ptio), the nNOS inhibitor (NPA) or the soluble guanylate cyclase inhibitor (ODQ) induced anxiolytic-like effects. The CB1 receptor antagonist AM251, but not the GABA(A) receptor antagonist bicuculline, attenuated the effect induced by AEA + c-Ptio combination. No effect, however, was found when anxiolytic doses of these same drugs were administered together. Combination of higher, ineffective doses of AEA and c-Ptio, NPA or ODQ was again anxiolytic. The effect of the former combination was prevented by low and ineffective doses of the GABA(A) receptor antagonist bicuculline or the GABA synthesis inhibitor L-allilglycine, suggesting that they depend on GABA(A)-mediated neurotransmission. AM251 was also able to attenuate this effect, indicating that in the presence of NO inhibition, the resultant anxiolytic-like effect could be due to AEA action on CBI receptors. The present results suggest that the AEA and nitrergic systems exert a complex functional interaction in the dlPAG to modulate anxiety behavior, probably interfering, in addition to glutamate, also with GABAergic mechanisms. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 07/06999-9 - Estudo da possível interação entre os sistemas canabinóide e nitrérgico na substância cinzenta periaquedutal dorsolateral na modulação de comportamentos defensivos em ratos
Beneficiário:Sabrina Francesca de Souza Lisboa
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/08705-8 - Avaliação da participação da via receptores NMDA/óxido nítrico no efeito ansiolítico observado pela ativação do sistema canabinoide da substância cinzenta periaquedutal dorsolateral de ratos submetidos ao modelo do labirinto em cruz elevado
Beneficiário:Amanda Conte Magesto
Linha de fomento: Bolsas no Brasil - Iniciação Científica