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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

COI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil

Texto completo
Autor(es):
Laurito, Magdalena [1] ; de Oliveira, Tatiane M. P. [2] ; Almiron, Walter Ricardo [1] ; Mureb Sallum, Maria Anice [2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Nacl Cordoba, Fac Ciencias Exactas Fis & Nat, Ctr Invest Entomol Cordoba, Inst Invest Biol & Tecnol, RA-5000 Cordoba - Argentina
[2] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01255 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Memórias do Instituto Oswaldo Cruz; v. 108, n. 1, p. 110-U152, DEC 2013.
Citações Web of Science: 40
Resumo

Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex (Culex) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species (Cx. acharistus, Cx. chidesteri, Cx. dolosus, Cx. lygrus and Cx. saltanensis) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus. Cx. maxi, Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex (Cux.) species. (AU)

Processo FAPESP: 11/20397-7 - Taxonomia morfológica e molecular e filogenia do subgênero Nyssorhynchus do gênero Anopheles (Diptera: Culicidae), com ênfase especial em Anopheles darlingi da Mata Atlântica
Beneficiário:Maria Anice Mureb Sallum
Linha de fomento: Auxílio à Pesquisa - Regular