Bovine herpesvirus type 5 infection regulates Bax/BCL-2 ratio

Autor(es)

Garcia, A. F. ^[1] ; Novais, J. B. ^[1] ; Antello, T. F. ^[1] ; Silva-Frade, C. ^[1] ; Ferrarezi, M. C. ^[1] ; Flores, E. F. ^[2] ; Cardoso, T. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Lab Virol Anim & Cult Celular, Fac Med Vet, Aracatuba, SP - Brazil

^[2] Univ Fed Santa Maria, Virol Lab, BR-97119900 Santa Maria, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 12, n. 3, p. 3897-3904, 2013.
• Citações Web of Science: 3

Resumo

Bovine herpesvirus 5 (BoHV-5) is an alpha-herpesvirus that causes neurological disease in young cattle and is also occasionally involved in reproductive disorders. Although there have been many studies of the apoptotic pathways induced by viruses belonging to the family Herpesviridae, there is little information about the intrinsic programmed cell death pathway in host-BoHV-5 interactions. We found that BoHV-5 is able to replicate in both mesenchymal and epithelial cell lines, provoking cytopathology that is characterized by cellular swelling and cell fusion. Viral antigens were detected in infected cells by immunofluorescence assay at 48 to 96 h post-infection (p.i.). At 48 to 72 h p.i., anti-apoptotic BCL-2 antigens were found at higher levels than Bax antigens; the latter is considered a pro-apoptotic protein. Infected cells had increased BCL-2 phenotype cells from 48 to 96 h p.i., based on flow cytometric analysis. At 48 to 96 h p.i., Bax mRNA was not expressed in any of the infected cell monolayers. In contrast, BCL-2 mRNA was found at high levels at all p.i. in both types of cells. BoHV-5 replication apparently modulates BCL-2 expression and gene transcription, enhancing production of virus progeny. (AU)

• Processo FAPESP: 09/17635-3 - Estudo epidemiológico, anátomo-patológico e molecular da meningoencefalite não supurativa ocasionada pelo herpesvírus tipo 5
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/50782-7 - Susceptibilidade de células embrionárias de perus (mesenquimais respiratórias e digestivas) à infecção in vitro pelo coronavírus tipo III
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular