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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Dendritic cell membrane CD83 enhances immune responses by boosting intracellular calcium release in T lymphocytes

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Autor(es):
Pinho, Mariana Pereira [1] ; Migliori, Isabella Katz [1] ; Flatow, Elizabeth Alexandra [1] ; Barbuto, Jose Alexandre M. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, BR-05508000 Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Journal of Leukocyte Biology; v. 95, n. 5, p. 755-762, MAY 2014.
Citações Web of Science: 12
Resumo

CD83 is a marker of mDCs directly related to their lymphostimulatory ability. Some data suggest that it has a central role in the immune system regulation, but how this function is performed remains to be determined. This work aimed to analyze the influence of CD83, present in mDCs, in the modulation of calcium signaling in T lymphocytes. Mo were differentiated into iDCs and activated with TNF-alpha. iDCs were treated, 4 h before activation, with siRNA(CD83), to reduce CD83 expression. Purified allogeneic T lymphocytes were labeled with the calcium indicator Fluo-4-AM, and calcium mobilization in the presence of mDCs was analyzed. CD83 knockdown mDCs induced lower calcium signal amplitude in T lymphocytes (29.0 +/- 10.0) compared with siRNA(scr)-treated mDCs (45.5 +/- 5.3). In another set of experiments, surface mDC CD83 was blocked with a specific mAb, and again, decreased calcium signaling in T lymphocytes was detected by flow cytometry and microscopy (fluorescence and confocal). In the presence of antibody, the percentage of responding T cells was reduced from 58.14% to 34.29%. As expected, anti-CD83 antibodies also reduced the proliferation of T lymphocytes (as assessed by CFSE dilution). Finally, in the absence of extracellular calcium, CD83 antibodies abrogated T cell signaling induced by allogeneic mDCs, suggesting that the presence of CD83 in mDC membranes enhances T lymphocyte proliferation by boosting calcium release from intracellular stores in these cells. Decreased availability of CD83 on the surface of dendritic cells decreases their ability to induce calcium mobilization in T lymphocytes. (AU)

Processo FAPESP: 09/54599-5 - Células dendríticas: elementos integrados do sistema imune - enfoque aplicado
Beneficiário:Jose Alexandre Marzagão Barbuto
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/01082-5 - Análise dos papéis da molécula de membrana CD83 na biologia da resposta imune
Beneficiário:Mariana Pereira Pinho
Linha de fomento: Bolsas no Brasil - Iniciação Científica