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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Reduced hippocampal manganese-enhanced MRI (MEMRI) signal during pilocarpine-induced status epilepticus: Edema or apoptosis?

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Autor(es):
Malheiros, Jackeline Moraes [1] ; Persike, Daniele Suzete [2] ; Cardoso de Castro, Leticia Urbana [3] ; Cunha Sanches, Talita Rojas [3] ; Andrade, Lucia da Conceicao [3] ; Tannus, Alberto [4] ; Covolan, Luciene [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, UNIFESP, Dept Fisiol, BR-0402306 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, UNIFESP, Dept Neurol & Neurocirurgia, BR-0402306 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med, Dept Nefrol, Sao Paulo - Brazil
[4] Univ Sao Paulo, IFSC, Ctr Imagens & Espectroscopia Vivo Ressonancia Mag, BR-13566590 Sao Carlos, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Epilepsy Research; v. 108, n. 4, p. 644-652, MAY 2014.
Citações Web of Science: 11
Resumo

Manganese-enhanced MRI (MEMRI) has been considered a surrogate marker of Ca+2 influx into activated cells and tracer of neuronal active circuits. However, the induction of status epilepticus (SE) by kainic acid does not result in hippocampal MEMRI hypersignal, in spite of its high cell activity. Similarly, short durations of status (5 or 15 min) induced by pilocarpine did not alter the hippocampal MEMRI, while 30 min of SE even reduced MEMRI signal Thus, this study was designed to investigate possible explanations for the absence or decrease of MEMRI signal after short periods of SE. We analyzed hippocampal caspase-3 activation (to evaluate apoptosis), T-2 relaxometry (tissue water content) and aquaporin 4 expression (water-channel protein) of rats subjected to short periods of pilocarpine-induced SE. For the time periods studied here, apoptotic cell death did not contribute to the decrease of the hippocampal MEMRI signal. However, T-2 relaxation was higher in the group of animals subjected to 30 min of SE than in the other SE or control groups. This result is consistent with higher AQP-4 expression during the same time period. Based on apoptosis and tissue water content analysis, the low hippocampal MEMRI signal 30 min after SE can potentially be attributed to local edema rather than to cell death. (C) 2014 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 05/56663-1 - Centro de imagens e espectroscopia in vivo por ressonância magnética para estudo de modelos animais
Beneficiário:Alberto Tannús
Linha de fomento: Auxílio à Pesquisa - Programa CINAPCE - Temático
Processo FAPESP: 07/52911-6 - Utilização da técnica de realce por manganês em ressonância magnética no estudo da epilepsia induzida por pilocarpina
Beneficiário:Jackeline Moraes Malheiros
Linha de fomento: Bolsas no Brasil - Mestrado