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Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System

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Autor(es):
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Sampaio, Suely C. F. [1] ; Moreira, Fabiana C. [1] ; Liberatore, Ana M. A. [2] ; Vieira, Monica A. M. [1] ; Knobl, Terezinha [3] ; Romao, Fabiano T. [1] ; Hernandes, Rodrigo T. [1, 4] ; Ferreira, Claudete S. A. [3] ; Ferreira, Antonio P. [3] ; Felipe-Silva, Aloisio [5] ; Sinigaglia-Coimbra, Rita [6] ; Koh, Ivan H. J. [2] ; Gomes, Tania A. T. [1]
Número total de Autores: 13
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Cirurgia, BR-04039032 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Vet, Dept Ornitopatol, BR-05508900 Sao Paulo - Brazil
[4] Univ Estadual Paulista, Inst Biociencias, Dept Microbiol & Imunol, BR-18618970 Sao Paulo - Brazil
[5] Fleury Med & Saude, BR-04344903 Sao Paulo - Brazil
[6] Univ Fed Sao Paulo, Escola Paulista Med, Ctr Microscopia Eletron, BR-04023062 Sao Paulo - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: BIOMED RESEARCH INTERNATIONAL; 2014.
Citações Web of Science: 1
Resumo

Atypical enteropathogenic Escherichia coli (aEPEC) inject various effectors into intestinal cells through a type three secretion system (T3SS), causing attaching and effacing (A/E) lesions. We investigated the role of T3SS in the ability of the aEPEC 1711-4 strain to interact with enterocytes in vitro (Caco-2 cells) and in vivo (rabbit ileal loops) and to translocate the rat intestinal mucosa in vivo. A T3SS isogenic mutant strain was constructed, which showed marked reduction in the ability to associate and invade but not to persist inside Caco-2 cells. After rabbit infection, only aEPEC 1711-4 was detected inside enterocytes at 8 and 24 hours pointing to a T3SS-dependent invasive potential in vivo. In contrast to aEPEC 1711-4, the T3SS-deficient strain no longer produced A/E lesions or induced macrophage infiltration. We also demonstrated that the ability of aEPEC 1711-4 to translocate through mesenteric lymph nodes to spleen and liver in a rat model depends on a functional T3SS, since a decreased number of T3SS mutant bacteria were recovered from extraintestinal sites. These findings indicate that the full virulence potential of aEPEC 1711-4 depends on a functional T3SS, which contributes to efficient adhesion/invasion in vitro and in vivo and to bacterial translocation to extraintestinal sites. (AU)

Processo FAPESP: 11/12664-5 - Explorando as interações de Escherichia coli enteropatogênica com células intestinais in vitro e in vivo
Beneficiário:Tânia Aparecida Tardelli Gomes do Amaral
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/53812-4 - Invasão: um mecanismo potencial de virulência em Escherichia coli enteropatogênica atípica?
Beneficiário:Tânia Aparecida Tardelli Gomes do Amaral
Linha de fomento: Auxílio à Pesquisa - Regular