Phylogenetic relationships of Leishmania species based on trypanosomatid barcode (SSU rDNA) and gGAPDH genes: Taxonomic revision of Leishmania (L.) infantum chagasi in South America

Autor(es):Mostrar menos -

Marcili, Arlei ^[1] ; Speranca, Marcia Ap. ^[2] ; da Costa, Andrea P. ^[1] ; Madeira, Maria de F. ^[3] ; Soares, Herbert S. ^[1] ; Sanches, Camila de O. C. C. ^[2] ; Acosta, Igor da C. L. ^[1] ; Girotto, Aline ^[1] ; Minervino, Antonio H. H. ^[1] ; Horta, Mauricio C. ^[4] ; Shaw, Jeffrey J. ^[5] ; Gennari, Solange M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet, Dept Med Vet Prevent & Saude Anim, BR-05508 Sao Paulo - Brazil

^[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil

^[3] Fundacao Oswaldo Cruz, Inst Pesquisa Clin Evandro Chagas, Rio De Janeiro, RJ - Brazil

^[4] Univ Fed Vale Sao Franscisco, Colegiado Med Vet, Petrolina, PE - Brazil

^[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INFECTION GENETICS AND EVOLUTION; v. 25, p. 44-51, JUL 2014.
• Citações Web of Science: 23

Resumo

Phylogenetic studies on trypanosomatid barcode using V7V8 SSU rRNA and gGAPDH gene sequences have provided support for redefining some trypanosomatid species and positioning new isolates. The genus Leishmania is a slow evolving monophyletic group and including important human pathogens. The phylogenetic relationships of this genus have been determined by the natural history of its vertebrate hosts, vector specificity, clinical manifestations, geographical distribution and molecular approaches using different markers. Thus, in an attempt to better understand the phylogenetic relationships of Leishmania species, we performed phylogenetic analysis on trypanosomatid barcode using V7V8 SSU rRNA and gGAPDH gene sequences among a large number of Leishmania species and also several Brazilian visceral Leishmania infantum chagasi isolates obtained from dogs and humans. Our phylogenetic analysis strongly suggested that Leishmania hertigi and Leishmania equatoriensis should be taxonomically revised so as to include them in the genus Endotrypanum; and supported ancient divergence of Leishmania enriettii. This, together with recent data in the literature, throws light on the discussion about the evolutionary southern supercontinent hypothesis for the origin of Leishmania ssp. and validates L. infantum chagasi from Brazil, thus clearly differentiating it from L. infantum, for the first time. (C) 2014 The Authors. Published by Elsevier B.V. (AU)

• Processo FAPESP: 10/50886-7 - Isolamento e caracterização morfológica, biológica e molecular e filogeografia multigênica de espécies causadoras de Leishmaniose visceral
• Beneficiário: Arlei Marcili
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/15311-6 - Diversidade de parasitas dos gêneros Leishmania e Trypanosoma em animais silvestres de uma unidade de conservação e animais domésticos do seu entorno no Estado do Espírito Santo.
• Beneficiário: Igor da Cunha Lima Acosta
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/19853-8 - Estudo Epidemiológico e Aspectos Ecológicos da Leishmaniose Visceral Canina no Estado do Maranhão
• Beneficiário: Andréa Pereira da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/20221-9 - Diagnóstico molecular e sorológico das espécies de Leishmania em amostras clínicas de pacientes e cães com leishmanose visceral do centro-oeste paulista
• Beneficiário: Marcia Aparecida Speranca
• Modalidade de apoio: Auxílio à Pesquisa - Regular