| Texto completo | |
| Autor(es): |
Messori, Luigi
[1]
;
Marzo, Tiziano
[1]
;
Fernandes Sanches, Rute Nazare
[2]
;
Hanif-Ur-Rehman
[2]
;
Silva, Denise de Oliveira
[2]
;
Merlino, Antonello
[3]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, FI - Italy
[2] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508000 Sao Paulo - Brazil
[3] Univ Naples Federico II, Dept Chem Sci, I-80126 Naples - Italy
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION; v. 53, n. 24, p. 6172-6175, JUN 2014. |
| Citações Web of Science: | 25 |
| Resumo | |
The reaction between the paddle-wheel tetrakis(acetato)chloridodiruthenium(II,III) complex, {[}Ru-2(mu-O2CCH3)(4)Cl] and hen egg-white lysozyme (HEWL) was investigated through ESI-MS and UV/Vis spectroscopy and the formation of a stable metal-protein adduct was unambiguously demonstrated. Remarkably, the diruthenium core is conserved in the adduct while two of the four acetate ligands are released. The crystal structure of this diruthenium-protein derivative was subsequently solved through X-ray diffraction analysis to 2.1 angstrom resolution. The structural data are in agreement with the solution results. It was found that HEWL binds two diruthenium moieties, at Asp101 and Asp119, respectively, with the concomitant release of two acetate ligands from each diruthenium center. (AU) | |
| Processo FAPESP: | 11/06592-1 - Metalofármacos anti-inflamatórios e antitumorais: reatividade, propriedades e interações com matrizes hospedeiras biocompatíveis |
| Beneficiário: | Denise de Oliveira Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |