Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Lipid oxidation induces structural changes in biomimetic membranes

Texto completo
Autor(es):
Mostrar menos -
Weber, Georges ; Charitat, Thierry [1] ; Baptista, Mauricio S. [2] ; Uchoa, Adjaci F. [2] ; Pavani, Christiane [2] ; Junqueira, Helena C. [3] ; Guo, Yachong [3] ; Baulin, Vladimir A. [4] ; Itri, Rosangela [3] ; Marques, Carlos M. [1] ; Schroder, Andre P. [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Strasbourg, Inst Charles Sadron, CNRS, F-67034 Strasbourg 2 - France
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Fis, Dept Fis Aplicada, BR-05314970 Sao Paulo - Brazil
[4] Univ Rovira & Virgili, Dept Engn Quim, E-43007 Tarragona - Spain
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: SOFT MATTER; v. 10, n. 24, p. 4241-4247, 2014.
Citações Web of Science: 39
Resumo

Oxidation can intimately influence and structurally compromise the levels of biological self-assembly embodied by intracellular and plasma membranes. Lipid peroxidation, a natural metabolic outcome of life with oxygen under light, is also a salient oxidation reaction in photomedicine treatments. However, the effect of peroxidation on the fate of lipid membranes remains elusive. Here we use a new photosensitizer that anchors and disperses in the membrane to achieve spatial control of the oxidizing species. We find, surprisingly, that the integrity of unsaturated unilamellar vesicles is preserved even for fully oxidized membranes. Membrane survival allows for the quantification of the transformations of the peroxidized bilayers, providing key physical and chemical information to understand the effect of lipid oxidation on protein insertion and on other mechanisms of cell function. We anticipate that spatially controlled oxidation will emerge as a new powerful strategy for tuning and evaluating lipid membranes in biomimetic media under oxidative stress. (AU)

Processo FAPESP: 12/50680-5 - Fotossensibilização nas ciências da vida
Beneficiário:Mauricio da Silva Baptista
Linha de fomento: Auxílio à Pesquisa - Temático