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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Maternal high-fat diet consumption modulates hepatic lipid metabolism and microRNA-122 (miR-122) and microRNA-370 (miR-370) expression in offspring

Texto completo
Autor(es):
Benatti, R. O. [1] ; Melo, A. M. [1] ; Borges, F. O. [1] ; Ignacio-Souza, L. M. [2] ; Simino, L. A. P. [1] ; Milanski, M. [1] ; Velloso, L. A. [3] ; Torsoni, M. A. [1] ; Torsoni, A. S. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Fac Ciencias Aplicadas, BR-13484350 Limeira, SP - Brazil
[2] Univ Fed Mato Grosso, Fac Nutricao, Cuiaba, Mato Grosso - Brazil
[3] Univ Estadual Campinas, Fac Ciencias Med, Dept Med Interna, Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BRITISH JOURNAL OF NUTRITION; v. 111, n. 12, p. 2112-2122, JUN 28 2014.
Citações Web of Science: 51
Resumo

Maternal consumption of a high-fat diet (HFD) during pregnancy and lactation is closely related to hepatic lipid accumulation, insulin resistance and increased serum cytokine levels in offspring and into their adulthood. MicroRNA (miRNA) have been implicated in cholesterol biosynthesis and fatty acid metabolism. We evaluated the modulation of hepatic fatty acid synthesis (de novo), beta-oxidation pathways, and miRNA-122 (miR-122) and miRNA-370 (miR-370) expression in recently weaned offspring (day 28) of mouse dams fed a HFD (HFD-O) or a standard chow (SC-O) during pregnancy and lactation. Compared with SC-O mice, HFD-O mice weighed more, had a larger adipose tissue mass and were more intolerant to glucose and insulin (P< 0.05). HFD-O mice also presented more levels of serum cholesterol, TAG, NEFA and hepatic I kappa B kinase and c-Jun N-terminal kinase phosphorylation compared with SC-O mice (P< 0.05). Protein levels of fatty acid synthase, acetyl-CoA carboxylase and 3-hydroxy-3-methylglutaryl-CoA reductase were similar in HFD-O and SC-O mice, whereas expression levels of SCD1 mRNA and protein were more abundant in HFD-O mice than in SC-O mice (P< 0.05). Interestingly, mRNA expression levels of the beta-oxidation-related genes ACADVL and CPT1 were decreased in HFD-O mice (P< 0.05). Furthermore, the expression of miR-122 was reduced but that of miR-370 was increased in HFD-O mice compared with that in SC-O mice (P< 0.05). Changes in hepatic lipid metabolism were accompanied by increased mRNA content of AGPAT1 and TAG deposition in HFD-O mice (P< 0.05). Taken together, the present results strongly suggest that maternal consumption of a HFD affects the early lipid metabolism of offspring by modulating the expression of hepatic beta-oxidation-related genes and miRNA that can contribute to metabolic disturbances in adult life. (AU)

Processo FAPESP: 11/13947-0 - Avaliação da expressão hepática de microRNAs relacionados ao metabolismo lipídico na prole de camundongos com obesidade induzida por dieta
Beneficiário:Rafaela Benatti de Oliveira
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 11/22156-7 - O consumo materno de dieta hiperlipídica durante a gestação e lactação e a modulação da expressão de microRNAs relacionados ao metabolismo de lipídeos
Beneficiário:Adriana Souza Torsoni
Linha de fomento: Auxílio à Pesquisa - Regular