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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases

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Autor(es):
M.A. Sukoyan ; A.Y. Kerkis ; M.R.B. Mello ; I.E. Kerkis ; J.A. Visintin ; L.V. Pereira
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Medical and Biological Research; v. 35, n. 5, p. 535-542, Maio 2002.
Resumo

Embryonic stem cells are totipotent cells derived from the inner cell mass of blastocysts. Recently, the development of appropriate culture conditions for the differentiation of these cells into specific cell types has permitted their use as potential therapeutic agents for several diseases. In addition, manipulation of their genome in vitro allows the creation of animal models of human genetic diseases and for the study of gene function in vivo. We report the establishment of new lines of murine embryonic stem cells from preimplantation stage embryos of 129/Sv mice. Most of these cells had a normal karyotype and an XY sex chromosome composition. The pluripotent properties of the cell lines obtained were analyzed on the basis of their alkaline phosphatase activity and their capacity to form complex embryoid bodies with rhythmically contracting cardiomyocytes. Two lines, USP-1 and USP-3, with the best in vitro characteristics of pluripotency were used in chimera-generating experiments. The capacity to contribute to the germ line was demonstrated by the USP-1 cell line. This cell line is currently being used to generate mouse models of human diseases. (AU)

Processo FAPESP: 98/10607-8 - Alexandre Kerkis | Academia de Ciências da Rússia - Rússia
Beneficiário:Lygia da Veiga Pereira
Linha de fomento: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
Processo FAPESP: 98/10703-7 - Irina Evgenievna Kerkis | Academia de Ciências da Rússia - Rússia
Beneficiário:Angela Maria Vianna Morgante
Linha de fomento: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
Processo FAPESP: 98/10606-1 - Marina Soukoian | Academia de Ciências da Rússia - Rússia
Beneficiário:Lygia da Veiga Pereira
Linha de fomento: Auxílio à Pesquisa - Pesquisador Visitante - Internacional