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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Association between EcoRI fragment-length polymorphism of the immunoglobulin lambda variable 8 (IGLV8) gene family with rheumatoid arthritis and systemic lupus erythematosus

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Autor(es):
R.G.P. Queiroz ; M.C. Tamia-Ferreira ; I.F. Carvalho ; F.C. Petean ; G.A.S. Passos
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Medical and Biological Research; v. 34, n. 4, p. 525-528, Abr. 2001.
Resumo

The human immunoglobulin lambda variable 8 (IGLV8) subgroup is a gene family containing three members, one of them included in a monomorphic 3.7-kb EcoRI genomic fragment located at the major lambda variable locus on chromosome 22q11.1 (gene IGLV8a, EMBL accession No. Z73650) at 100% frequency in the normal urban population. The second is a polymorphic RFLP allele included in a 6.0-kb EcoRI fragment at 10% frequency, and the third is located in a monomorphic 8.0-kb EcoRI fragment at 100% frequency, the last being translocated to chromosome 8q11.2 and considered to be an orphan gene. Our Southern blot-EcoRI-RFLP studies in normal individuals and in patients with rheumatoid arthritis (RA) or with systemic lupus erythematosus (SLE), using a specific probe for the IGLV8 gene family (probe pVL8, EMBL accession No. X75424), have revealed the two monomorphic genomic fragments containing the IGLV8 genes, i.e., the 3.7-kb fragment from chromosome 22q11.1 and the 8.0-kb fragment from 8q11.2, both occurring at 100% frequency (103 normal individuals, 48 RA and 28 SLE patients analyzed), but absence of the 6.0-kb IGLV8 polymorphic RFLP allele in all RA or SLE patients. As expected, the frequency of the 6.0-kb allele among the normal individuals was 10%. These findings suggest an association between the absence of the 6.0-kb EcoRI fragment and rheumatoid arthritis and systemic lupus erythematosus. (AU)

Processo FAPESP: 98/09789-4 - Emergência da recombinação V(D)J nos locus TCRbeta e expressão gênica diferencial durante a ontogenia do timo
Beneficiário:Geraldo Aleixo da Silva Passos Júnior
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 98/05584-9 - Sequenciamento, recombinação e expressão de genes V-lambda das imunoglobulinas humanas no lúpus eritematoso sistêmico
Beneficiário:Geraldo Aleixo da Silva Passos Júnior
Linha de fomento: Auxílio à Pesquisa - Regular