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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from Sao Paulo State, Brazil

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Autor(es):
Porfida Ferreira, Thais Sebastiana [1] ; Moreno, Andrea Micke [2] ; de Almeida, Renata Rodrigues [1] ; Gomes, Cleise Ribeiro [1] ; Sena de Gobbi, Debora Dirani [1] ; Nogueira de Lima Filsner, Pedro Henrique [1] ; Moreno, Marina [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, FMVZ, Dept Med Vet Prevent & Sande Anim VPS, Lab Sanidade Suina & Virol, Programa Posgrad Epide, BR-05508270 Sao Paulo - Brazil
[2] Univ Sao Paulo, FMVZ, VPS, Lab Sanidade Suina & Virol, BR-05508270 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Ciência Rural; v. 42, n. 8, p. 1450-1456, AUG 2012.
Citações Web of Science: 3
Resumo

Clostridium perfringens is an anaerobic Gram-positive bacterium known as common pathogen for humans, for domestic and wildlife animals. Although infections caused by C. perfringens type C and A in swine are well studied, just a few reports describe the genetic relationship among strains in the epidemiological chain of swine clostridioses, as well as the presence of the microorganism in the slaughterhouses. The aim of the present study was to isolate C. perfringens from feces and carcasses from swine slaughterhouses, characterize the strains in relation to the presence of enterotoxin, alpha, beta, epsilon, iota and beta-2 toxins genes. using polymerase chain reaction (PCR) and comparing strains by means of Pulsed field gel electrophoresis (PFGE). Clostridium perfringens isolation frequencies in carcasses and finishing pig intestines were of 58.8% in both types of samples. According to the polymerase chain reaction assay, only, alfa toxin was detected, being all isolates also negative to enterotoxin and beta2 toxin. Through PFGE technique, the strains were characterized in 35 pulsotypes. In only one pulsotype, the isolate from carcass sample was grouped with fecal isolate of the same animal, suggesting that the risk of cross-contamination was low. Despite the high prevalence of C. perfringens in swine carcasses from the slaughterhouses assessed, the risk of food poisoning to Brazilian pork consumers is low, since all strains were negative to cpe-gene, codifying enterotoxin. (AU)

Processo FAPESP: 03/01645-3 - Caracterização genotípica de amostras de Clostridium perfringens provenientes de suínos através da PCR-ribotipagem, do polimorfismo do comprimento de fragmentos amplificados (AFLP) e da eletroforese em gel de campo pulsado (PFGE)
Beneficiário:Andrea Micke Moreno
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 04/04024-2 - Caracterização genotípica de isolados de Clostridium perfringens provenientes de suínos, através da PCR-ribotipagem
Beneficiário:Renata Rodrigues de Almeida
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 04/04023-6 - Caracterização genotípica de amostras de Clostridium perfringens de origem suína através de eletroforese em campo pulsado (PFGE)
Beneficiário:Thaís Sebastiana Porfida Ferreira
Linha de fomento: Bolsas no Brasil - Mestrado