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Proteomic analyses of baculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus budded and occluded virus

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Autor(es):
Braconi, Carla Torres [1] ; Pereira Ardisson-Araujo, Daniel Mendes [2] ; Paes Leme, Adriana Franco [3] ; de Castro Oliveira, Juliana Velasco [1] ; Pauletti, Bianca Alves [3] ; Garcia-Maruniak, Alejandra [4] ; Ribeiro, Bergmann Morais [2] ; Maruniak, James E. [4] ; de Andrade Zanotto, Paolo Marinho [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Lab Mol Evolut & Bioinformat, Dept Microbiol, Biomed Sci Inst ICB 2, Sao Paulo - Brazil
[2] Univ Brasilia, Cell Biol Dept, BR-70910970 Brasilia, DF - Brazil
[3] Brazilian Biosci Natl Lab LNBio CNPEM, Lab Mass Spectrometry, Campinas, SP - Brazil
[4] Univ Florida, Insect Virol Lab, Dept Entomol & Nematol, Gainesville, FL 32611 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF GENERAL VIROLOGY; v. 95, n. 4, p. 980-989, APR 2014.
Citações Web of Science: 14
Resumo

Baculoviruses infect insects, producing two distinct phenotypes during the viral life cycle: the budded virus (By) and the occlusion-derived virus (ODV) for intra- and inter-host spread, respectively. Since the 1980s, several countries have been using Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) as a biological control agent against the velvet bean caterpillar, A. gemmatalis. The genome of AgMNPV isolate 2D (AgMNPV-2D) carries at least 152 potential genes, with 24 that possibly code for structural proteins. Proteomic studies have been carried out on a few baculoviruses, with six ODV and two BV proteomes completed so far. Moreover, there are limited data on virion proteins carried by AgMNPV-2D. Therefore, structural proteins of AgMNPV-2D were analysed by MALDI- quadrupole-TOF and liquid chromatography MS/MS. A total of 44 proteins were associated with the ODV and 33 with the BV of AgMNPV-2D. Although 38 structural proteins were already known, we found six new proteins in the ODV and seven new proteins carried by the AgMNPV-2D BV. Eleven cellular proteins that were found on several other enveloped viruses were also identified, which are possibly carried with the virion. These findings may provide novel insights into baculovirus biology and their host interaction. Moreover, our data may be helpful in subsequent applied studies aiming to improve AgMNPV use as a biopesticide and a biotechnology tool for gene expression or delivery. (AU)

Processo FAPESP: 08/10713-6 - Estudo das proteínas estruturais e a caracterização da proteina codificada pela ORF-31 do Nucleopoliedrovírus Anticarsia gemmatalis (AgMNPV-2D) em linhagens de células de inseto distintas
Beneficiário:Paolo Marinho de Andrade Zanotto
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/16740-8 - Estudo das proteínas estruturais do nucleopoliedrovírus Anticarsia gemmatalis (AgMNPV-2D) em linhagens de células de inseto distintas e comparação da GP64 em variantes geográficas
Beneficiário:Carla Torres Braconi
Linha de fomento: Bolsas no Brasil - Doutorado Direto