| Texto completo | |
| Autor(es): Mostrar menos - |
Ferraz, Miriele Cristina
[1]
;
Celestino Parrilha, Luciane Aparecida
[2]
;
Duarte Moraes, Maria Silvia
[3]
;
Amaral Filho, Jorge
[4]
;
Cogo, Jose Carlos
[5]
;
dos Santos, Marcio Galdino
[6]
;
Franco, Luiz Madaleno
[7]
;
Groppo, Francisco Carlos
[8]
;
Puebla, Pilar
[9]
;
San Feliciano, Arturo
[10]
;
Oshima-Franco, Yoko
[11]
Número total de Autores: 11
|
| Afiliação do(s) autor(es): Mostrar menos - | [1] Univ Sorocaba. UNISO
[2] Univ Sorocaba. UNISO
[3] Univ Sorocaba. UNISO
[4] Univ Sorocaba. UNISO
[5] Univ Vale Paraiba. UNIVAP
[6] Fed Univ Tocantins. UFT
[7] Univ Metodista Piracicaba. UNIMEP
[8] State Univ Campinas UNICAMP. Piracicaba Dent Sch
[9] Univ Salamanca. USAL
[10] Univ Salamanca. USAL
[11] Univ Sorocaba. UNISO
Número total de Afiliações: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CURRENT ORGANIC CHEMISTRY; v. 16, n. 22, p. 2717-2723, NOV 2012. |
| Citações Web of Science: | 8 |
| Resumo | |
Traditional medicine of Brazilian central savannah preconizes Dipteryx alata extracts against snakebites venoms. Previous studies have shown the extract of this plant's bark antagonizing the neuromuscular blockade caused by snake venom in isolated mice diaphragm and phrenic nerve models. In the present study, the triterpenoids lupeol, lupenone, 28-OH-lupenone and betulin, which are constituents of the plant, were observed by using in vitro pharmacological tests. These tests showed the efficacy of all tested triterpenoids (1 mg/5mL) against the irreversible neuromuscular blockade caused by snake venom from Bothrops jararacussu (Bjssu, 40 g/mL, n=19), as following: betulin (68 +/- 7%, n=11) +/- lupeol (70 +/- 8%, n=5) > lupenone (45 +/- 9%, n=7) similar to 28-OH-lupenone (54 +/- 5%, n=4). Betulin (39.5 +/- 9%, n=9) and lupenone (49.5 +/- 8%, n=4) significantly protected against Crotalus durissus terrificus (CDT, 10 g/mL, n=10) envenomation of neuromuscular junction. The mixture of betulin or lupeol + Bjssu venom and betulin or lupenone + CDT venom showed significant phytochemical protection in morphological analysis. Considering both pharmacological and morphological results, betulin showed the best protective effect against neuromuscular blockade and myotoxicity caused by both snake venoms. In addition, it has a potent short-term excitatory action in the neuromuscular junction. We concluded that the studied triterpenoids, especially betulin, showed antiophidian properties. (AU) | |
| Processo FAPESP: | 04/09705-8 - Investigacao farmacologica da atividade antipeconhenta de fracoes obtidas de extratos de casearia sylvestris sw. |
| Beneficiário: | Yoko Oshima Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 08/52643-4 - Estudo da capacidade neutralizante do extrato hidroalcoolico de camellia sinensis (cha verde) contra os efeitos neurotoxico e miotoxico da bothropstoxina-i (bothrops jararacussu). |
| Beneficiário: | Yoko Oshima Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 07/53883-6 - Dipteryx alata vogel: estudo da acao antiofidica e da toxicidade. |
| Beneficiário: | Yoko Oshima Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 08/11005-5 - Dipteryx alata Vogel: estudos da ação antiofídica e de toxicidade |
| Beneficiário: | Yoko Oshima Franco |
| Modalidade de apoio: | Bolsas no Exterior - Pesquisa |