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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Detection of Streptococcus agalactiae colonization in pregnant women by using combined swab cultures: cross-sectional prevalence study

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Autor(es):
Marconi, Camila [1] ; Rocchetti, Talita Trevizani [1] ; Mores Rall, Vera Lucia [2] ; de Carvalho, Lidia Raquel [3] ; Medeiros Borges, Vera Terezinha [4] ; da Silva, Marcia Guimaraes [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Dept Pathol, Fac Med Botucatu, Sao Paulo - Brazil
[2] Univ Estadual Paulista, Dept Microbiol & Immunol, Inst Biosci, Sao Paulo - Brazil
[3] Univ Estadual Paulista, Dept Biostat, Inst Biosci, Sao Paulo - Brazil
[4] Univ Estadual Paulista, Dept Gynecol & Obstet, Fac Med Botucatu, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: São Paulo Medical Journal; v. 128, n. 2, p. 60-62, MAR 4 2010.
Citações Web of Science: 5
Resumo

CONTEXT AND OBJECTIVE: Maternal Streptococcus agalactiae colonization and early-onset neonatal sepsis have aroused interest in the worldwide literature. Streptococcal neonatal disease is associated with significant morbidity and mortality in the perinatal period, especially among premature neonates. The aim of this study was to assess the prevalence of maternal streptococcal colonization by using combined swab cultures, compared with swab collection from a single site. DESIGN AND SETTING: Cross-sectional study at Faculdade de Medicine de Botucatu, Universidade Estadual Paulista. METHODS: Samples were obtained from 405 patients at gestational ages of 35 to 37 weeks. Swabs from the perianal (rectal) region, vaginal introitus and upper lateral vaginal vault were cultured in Todd-Hewitt selective broth. Colonies suggestive of Streptococcus agalactiae were subjected to the catalase and CAMP (Christie, Atkins, Munch-Petersen) tests. To evaluate the positivity of combined swab cultures, Tukey's test was used for comparison of proportions. RESULTS: The prevalence of streptococcal colonization was 25.4%. Among the patients with positive cultures, 28.1% had this at only one collection site, 24.2% simultaneously at two sites and 47.5% at all three sites. Associating the swabs from two collection sites significantly increased streptococcal isolation, compared with a single swab (P < 0.05), except for perianal (rectal) collection. Use of combined swabs from three collection sites showed statistically higher isolation rates. CONCLUSION: In combined swab cultures collected from three collection sites, the prevalence of maternal Streptococcus agalactiae colonization was higher than in swabs collected from a single site. (AU)

Processo FAPESP: 07/51704-7 - Incidência de colonização retovaginal por Streptococcus agalactiae e associação com a microbiota vaginal em gestantes atendidas no Hospital das Clínicas da Faculdade de medicina de Botucatu, UNESP
Beneficiário:Márcia Guimarães da Silva
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 06/55307-0 - Incidência de colonização retovaginal por Streptococcus agalactiae e associação com a microbiologia vaginal em gestantes atendidas no Hospital das Clínicas da Faculdade de Medicina de Botucatu, UNESP
Beneficiário:Talita Trevizani Rocchetti
Linha de fomento: Bolsas no Brasil - Iniciação Científica