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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genomic and phenotypic attributes of novel salinivibrios from stromatolites, sediment and water from a high altitude lake

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Autor(es):
Gorriti, Marta F. [1, 2] ; Dias, Graciela M. [1] ; Chimetto, Luciane A. [1] ; Trindade-Silva, Amaro E. [1, 3] ; Silva, Bruno S. [1] ; Mesquita, Milene M. A. [1] ; Gregoracci, Gustavo B. [1] ; Farias, Maria E. [2] ; Thompson, Cristiane C. [1] ; Thompson, Fabiano L. [1, 4, 5]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Fed Rio de Janeiro, Inst Biol, Microbiol Lab, Rio De Janeiro - Brazil
[2] Consejo Nacl Invest Cient & Tecn, CCT, Planta Piloto Proc Ind Microbiol PROIMI, Lab Invest Microbiol Lagunas Andinas, San Miguel De Tucuman, Tucuman - Argentina
[3] Fac Tecnol & Ciencias, Nucleo Biotecnol Ambiental, Salvador, BA - Brazil
[4] SAGE COPPE, Rio De Janeiro - Brazil
[5] Microbiol Lab, CCS, IB, Rio De Janeiro, RJ - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: BMC Genomics; v. 15, JUN 13 2014.
Citações Web of Science: 20
Resumo

Background: Salinivibrios are moderately halophilic bacteria found in salted meats, brines and hypersaline environments. We obtained three novel conspecific Salinivibrio strains closely related to S. costicola, from Socompa Lake, a high altitude hypersaline Andean lake (approx. 3,570 meters above the sea level). Results: The three novel Salinivibrio spp. were extremely resistant to arsenic (up to 200 mM HAsO4(2-)), NaCl (up to 15%), and UV-B radiation (19 KJ/m(2), corresponding to 240 minutes of exposure) by means of phenotypic tests. Our subsequent draft genome ionsequencing and RAST-based genome annotation revealed the presence of genes related to arsenic, NaCl, and UV radiation resistance. The three novel Salinivibrio genomes also had the xanthorhodopsin gene cluster phylogenetically related to Marinobacter and Spiribacter. The genomic taxonomy analysis, including multilocus sequence analysis, average amino acid identity, and genome-to-genome distance revealed that the three novel strains belong to a new Salinivibrio species. Conclusions: Arsenic resistance genes, genes involved in DNA repair, resistance to extreme environmental conditions and the possible light-based energy production, may represent important attributes of the novel salinivibrios, allowing these microbes to thrive in the Socompa Lake. (AU)

Processo FAPESP: 09/11612-1 - Caracterização do grupamento gênico de biossíntese de Arenosclerinas, alcaloides alquilpiperidínicos isolados da esponja Arenosclera brasiliensis
Beneficiário:Amaro Emiliano Trindade Silva
Linha de fomento: Bolsas no Brasil - Pós-Doutorado