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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome

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Autor(es):
Aitken, Elizabeth H. [1] ; Negri, Elnara M. [2] ; Barboza, Renato [3] ; Lima, Maria Ri [1] ; Alvarez, Jose M. [1] ; Marinho, Claudio R. F. [4] ; Caldini, Elia G. [2] ; Epiphanio, Sabrina [1, 5]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Immunol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Lab Med Invest 59, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Phys & Earth Sci, Diadema - Brazil
[4] Univ Sao Paulo, Dept Parasitol, Sao Paulo - Brazil
[5] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Malaria Journal; v. 13, JUN 13 2014.
Citações Web of Science: 13
Resumo

Background: The mechanisms through which infection with Plasmodium spp. result in lung disease are largely unknown. Recently a number of mouse models have been developed to research malaria-associated lung injury but no detailed ultrastructure studies of the disease in its terminal stages in a murine model have yet been published. The goal was to perform an ultrastructural analysis of the lungs of mice that died with malaria-associated acute lung injury/acute respiratory distress syndrome to better determine the relevancy of the murine models and investigate the mechanism of disease. Methods: DBA/2 mice were infected with Plasmodium berghei strain ANKA. Mice had their lungs removed immediately after death, processed using standard methods and viewed by transmission electron microscopy (TEM). Results: Infected red blood cell: endothelium contact, swollen endothelium with distended cytoplasmic extensions and thickening of endothelium basement membrane were observed. Septa were thick and filled with congested capillaries and leukocytes and the alveolar spaces contained blood cells, oedema and cell debris. Conclusion: Results show that the lung ultrastructure of P. berghei ANKA-infected mice has similar features to what has been described in post-mortem TEM studies of lungs from individuals infected with Plasmodium falciparum. These data support the use of murine models to study malaria-associated acute lung injury. (AU)

Processo FAPESP: 09/53889-0 - Estudo dos mecanismos imunopatológicos envolvidos na malária durante a gravidez
Beneficiário:Cláudio Romero Farias Marinho
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 09/53256-7 - Identificação e caracterização da síndrome respiratória aguda murina associada à malária severa: estudo da interação parasito-hospedeiro
Beneficiário:Sabrina Epiphanio
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores