Can hexanoic acid signaling modulate the transcriptome, metabolome and pathogen development in coffee trees?

• Grant number: 16/10896-0
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: October 01, 2016
• End date: October 31, 2021
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Douglas Silva Domingues
• Grantee: Douglas Silva Domingues
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated researchers: Alexandre Rossi Paschoal ; Danilo da Cruz Centeno ; Emerson Alves da Silva ; Flávia Rodrigues Alves Patrício ; Gustavo Habermann ; Halley Caixeta de Oliveira ; José Domingos Cochicho Ramalho ; Luiz Filipe Protasio Pereira ; Milene Ferro ; Mirian Perez Maluf ; Poliana Cardoso Gustavson ; Silvia Ribeiro de Souza
• Associated scholarship(s): 17/01455-2 - Biochemical and molecular basis of the metabolic network of diterpenes, bioactive and natural compounds in coffee, BP.PD

Abstract

Arabica coffee (Coffea arabica L.) is an allotetraploid in which large scale studies related to signaling molecules are still neglected, despite the economic and social importance of its cultivation in Brazil and worldwide. Recently, it was shown that hexanoic acid, a hexane-derivative fatty acid, can act both as a resistance inducer (primer) molecule and as an antimicrobial substance in several plant systems. This priming probably relies on the modulation of jasmonate, salicylate and mevalonate metabolism, pathways also related to the regulation of terpene biosynthesis, the largest class of specialized metabolites produced in plants. Only plants from Coffea genus produces the antioxidant and anticarcinogenic diterpenes cafestol and kahweol, whose molecular basis of biosynthesis is poorly known. We recently detected these diterpenes, for the first time, in coffee roots. So far, molecular mechanisms modulated by the hexanoic acid application were studied almost exclusively on plant/pathogen interactions, and the effect per se of hexanoic acid on plant metabolism was not specifically evaluated. In this project, we will verify if the exogenous application of hexanoic acid is able to modulate transcriptional profiles, the primary metabolism and terpenoid profiles in Arabica coffee leaves and roots. We will also analyze if this substance can modify antioxidant system responses and nitric oxide production in coffee plants. In addition, we will check if hexanoic acid is also able to inhibit the development of brown eye spot and brown leaf spot in coffee plants. Thus, this project will produce knowledge on several areas, which cover the molecular basis of terpenoid metabolism in plants and the control of diseases without resistance sources in coffee trees. Concomitantly, this project will bring fundamental knowledge to elucidate the relevance of hexanoic acid application on molecular networks related to nitric oxide signaling and stress tolerance in plants (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GAVASSI, MARINA ALVES; SILVA, GISELLE SCHWAB; SANTIAGO DA SILVA, CAROLINA DE MARCHI; THOMPSON, ANDREW J.; MACLEOD, KYLE; RAYNER OLIVEIRA, PAULO MARCELO; CAVALHEIRO, MARIANA FEITOSA; DOMINGUES, DOUGLAS SILVA; HABERMANN, GUSTAVO. CED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stres. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 185, MAY 2021. (15/25409-4, 17/26144-0, 16/10896-0, 18/25658-2, 18/08902-7)

CACCALANO, M. N.; DILARRI, G.; ZAMUNER, C. F. C.; DOMINGUES, D. S.; FERREIRA, H.. Hexanoic acid: a new potential substitute for copper-based agrochemicals against citrus canker. Journal of Applied Microbiology, v. 131, n. 5, p. 2488-2499, NOV 2021. (16/10896-0, 15/50162-2, 14/50880-0, 17/50216-0, 17/07306-9)

BUDZINSKI, ILARA G. F.; CAMARGO, PAULA O.; LEMOS, SAMARA M. C.; GUYOT, ROMAIN; CALZADO, NATALIA F.; IVAMOTO-SUZUKI, SUZANA T.; DOMINGUES, DOUGLAS S.. Transcriptomic alterations in roots of two contrasting Coffea arabica cultivars after hexanoic acid priming. FRONTIERS IN GENETICS, v. 13, p. 5-pg., 2022-10-28. (16/10896-0)

DE LEMOS, SAMARA MIREZA CORREIA; PASCHOAL, ALEXANDRE ROSSI; GUYOT, ROMAIN; MEDEMA, MARNIX; DOMINGUES, DOUGLAS SILVA. Genome mining of metabolic gene clusters in the Rubiaceae family. COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, v. 23, p. 12-pg., 2023-12-08. (18/08042-8, 16/10896-0)

SALOJAERVI, JARKKO; RAMBANI, ADITI; YU, ZHE; GUYOT, ROMAIN; STRICKLER, SUSAN; LEPELLEY, MAUD; WANG, CUI; RAJARAMAN, SITARAM; RASTAS, PASI; ZHENG, CHUNFANG; et al. The genome and population genomics of allopolyploid Coffea arabica reveal the diversification history of modern coffee cultivars. Nature Genetics, v. 56, n. 4, p. 27-pg., 2024-04-01. (17/01455-2, 16/10896-0)

CHIQUITTO, ALISSON G.; SILVA, LUCAS OTAVIO L.; OLIVEIRA, LILIANE SANTANA; DOMINGUES, DOUGLAS S.; PASCHOAL, ALEXANDRE R.. Make No Mistake! Why Do Tools Make Incorrect Long Non-coding RNA Classification?. ADVANCES IN BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, BSB 2023, v. 13954, p. 10-pg., 2023-01-01. (18/08042-8, 19/15477-3, 16/10896-0)

GAVASSI, MARINA ALVES; SILVA, GISELLE SCHWAB; SANTIAGO DA SILVA, CAROLINA DE MARCHI; THOMPSON, ANDREW J.; MACLEOD, KYLE; RAYNER OLIVEIRA, PAULO MARCELO; CAVALHEIRO, MARIANA FEITOSA; DOMINGUES, DOUGLAS SILVA; HABERMANN, GUSTAVO. NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 185, p. 12-pg., 2021-02-06. (16/10896-0, 18/08902-7, 17/26144-0, 18/25658-2, 15/25409-4)

CAVALHEIRO, MARIANA F.; GAVASSI, MARINA A.; SILVA, GISELLE S.; NOGUEIRA, MATHEUS A.; SILVA, CAROLINA M. S.; DOMINGUES, DOUGLAS S.; HABERMANN, GUSTAVO. Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium. FUNCTIONAL PLANT BIOLOGY, v. 47, n. 2, p. 10-pg., 2020-01-01. (16/10896-0, 13/11370-3, 17/26144-0, 15/25409-4)

DE OLIVEIRA, FERNANDA FREITAS; TOMAZ, JUAREZ PIRES; DA SILVA, BRUNA SILVESTRE RODRIGUES; DOS SANTOS, TIAGO BENEDITO; IVAMOTO-SUZUKI, SUZANA TIEMI; SCHOLZ, MARIA BRIGIDA DOS SANTOS; PEREIRA, LUIZ FILIPE PROTASIO. Coffea arabica L. genes from isoprenoid metabolic pathways are more expressed in full sun cultivation systems than in agroforestry systems. PLANT GENE, v. 26, p. 6-pg., 2021-06-01. (16/10896-0, 17/01455-2)

IVAMOTO-SUZUKI, SUZANA TIEMI; CELEDON, JOSE MIGUEL; YUEN, MACAIRE M. S.; KITZBERGER, CI; SILVA DOMINGUES, DOUGLAS; BOHLMANN, JORG; PROTASIO PEREIRA, LUIZ FILIPE. Functional Characterization of ent-Copalyl Diphosphate Synthase and Kaurene Synthase Genes from Coffea arabica L. Journal of Agricultural and Food Chemistry, v. 71, n. 42, p. 11-pg., 2023-10-10. (17/01455-2, 16/10896-0)

CAVALHEIRO, MARIANA F.; GAVASSI, MARINA A.; SILVA, GISELLE S.; NOGUEIRA, MATHEUS A.; SILVA, CAROLINA M. S.; DOMINGUES, DOUGLAS S.; HABERMANN, GUSTAVO. Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in `Rangpur' lime plants exposed to aluminium. FUNCTIONAL PLANT BIOLOGY, v. 47, n. 2, p. 112-121, 2020. (15/25409-4, 13/11370-3, 17/26144-0, 16/10896-0)

BABA, VIVIANE YUMI; BRAGHINI, MASAKO TOMA; DOS SANTOS, TIAGO BENEDITO; DE CARVALHO, KENIA; MOURA SOARES, JOAO DANILLO; IVAMOTO-SUZUKI, SUZANA TIEMI; MALUF, MIRIAN P.; PADILHA, LILIAN; PACCOLA-MEIRELLES, LUZIA D.; PEREIRA, LUIZ FILIPE; et al. Transcriptional patterns of Coffea arabica L. nitrate reductase, glutamine and asparagine synthetase genes are modulated under nitrogen suppression and coffee leaf rust. PeerJ, v. 8, JAN 3 2020. (17/01455-2, 16/10896-0)

COSTA-SILVA, JULIANA; HUNGRIA, MARIANGELA; DOMINGUES, DOUGLAS S.; MENOTTI, DAVID; LOPES, FABRICIO MARTINS. Temporal progress of gene expression analysis with RNA-Seq data: A review on the relationship between computational methods. COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, v. 21, p. 13-pg., 2023-01-01. (18/08042-8, 16/10896-0, 19/15477-3)

BUDZINSKI, ILARA G. F.; CAMARGO, PAULA O.; ROSA, RAISSA S.; CALZADO, NATALIA F.; IVAMOTO-SUZUKI, SUZANA T.; DOMINGUES, DOUGLAS S.. Transcriptome Analyses of Leaves Reveal That Hexanoic Acid Priming Differentially Regulate Gene Expression in Contrasting Coffea arabica Cultivars. FRONTIERS IN SUSTAINABLE FOOD SYSTEMS, v. 5, OCT 25 2021. (16/10896-0, 17/01455-2)

SILVA, NATACHA; IVAMOTO-SUZUKI, SUZANA TIEMI; CAMARGO, PAULA OLIVEIRA; ROSA, RAISSA SCALZONI; PROTASIO PEREIRA, LUIZ FILIPE; DOMINGUES, DOUGLAS SILVA. Low-Copy Genes in Terpenoid Metabolism: The Evolution and Expression of MVK and DXR Genes in Angiosperms. PLANTS-BASEL, v. 9, n. 4, APR 2020. (17/01455-2, 16/10896-0)