Investigation of the effects of combined training in the premature immunosenescence associated with obesity

• Grant number: 17/06643-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2017
• End date: September 30, 2020
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Claudia Regina Cavaglieri
• Grantee: Diego Trevisan Brunelli
• Host Institution: Faculdade de Educação Física (FEF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The present study aims: to verify the gene expression of pro-and anti-inflammatory markers related to premature immunosenescence associated with obesity in healthy and obese middle-aged adult individuals; to investigate the effects of regular physical exercise on the gene expression of pro-and anti-inflammatory markers related to the premature immunosenescence associated with obesity in obese middle-aged adult individuals. The sample will be composed of middle-aged obese individuals (body mass index (BMI) 30 -35 kg/m²) diabetic type 2, of both sexes, who will be distributed in Control Group (CG) and Combined Training group (CT). In addition, a group of volunteers of the same age and healthy (BMI 20 - 25 kg / m²) will be used as a Healthy Control Group (HCG) to compare the variables at the initial moment. The functional measurements (muscular strength and cardiorespiratory fitness), body composition (plethysmography), biochemical (serum concentrations of leptin, IL-2, IL-4, IL-6, IL-10, immunoglobulin G (IgG), TNF-±, Anti-CMV and anti-EBV) and molecular (leptin, IL-2, IL-4, IL-6, IL-10, and TNF- ± gene expression of leukocytes and adipose tissue) will be performed before (pre) and after (post) the experimental period. The CT program will be composed of strength training (30 min, 1-3 sets of 8 to 12 repetitions, 50-75% of 1RM, 1-1 min. and 30 sec. of interval) followed aerobic training (35 min. at 50-60% VO2max) in the same session, performed 3 times weekly for 16 weeks. The CT program will consist of performing strength training (~ 30 min, 1-3 sets of 8 to 12 repetitions, 50 to 75% of 1RM, 1 to 1 min and 30 sec interval) followed by aerobic training (35 min - 50-60% of VO2max) in the same session, performed 3 times a week for 16 weeks. The following hypotheses are expected to be observed: obese middle-aged adults may present in the circulating blood, adipose tissue and leukocytes a greater expression of leptin and other immunosenescence-related pro-inflammatory markers when compared to eutrophic adults of the same Age, thus indicating the presence of an premature immunosenescence associated with obesity; Regular physical exercise, especially CT, may promote decreases in the gene expression of pro-inflammatory markers related to the premature immunosenescence associated with obesity, thus contributing to the reestablishment of an adequate control of the immune functions in obese middle-aged individuals. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NASCIMENTO, EDUARDO MARCEL FERNANDES; BORSZCZ, FERNANDO KLITZKE; VENTURA, THIAGO PEREIRA; CAPUTO, FABRIZIO; GUGLIELMO, LUIZ GUILHERME ANTONACCI; DE LUCAS, RICARDO DANTAS. Reliability and Validity of Cycling Sprint Performance at Isolinear Mode Without Torque Factor: A Preliminary Study in Well-Trained Male Cyclists. RESEARCH QUARTERLY FOR EXERCISE AND SPORT, v. 95, n. 3, p. 8-pg., 2024-02-07. (17/06643-1, 16/08751-3)

BRUNELLI, DIEGO T.; BONFANTE, IVAN L. P.; BOLDRINI, VINICIUS O.; SCOLFARO, PEDRO G.; DUFT, RENATA G.; MATEUS, KERYMA; FATORI, RAPHAEL F.; CHACON-MIKAHIL, MARA P. T.; FARIAS, ALESSANDRO S.; TEIXEIRA, ANA M.; et al. Combined Training Improves Gene Expression Related to Immunosenescence in Obese Type 2 Diabetic Individuals. RESEARCH QUARTERLY FOR EXERCISE AND SPORT, v. 95, n. 3, p. 10-pg., 2024-02-08. (17/06643-1, 16/08751-3)

BRUNELLI, DIEGO T.; BOLDRINI, VINICIUS O.; BONFANTE, IVAN L. P.; DUFT, RENATA G.; MATEUS, KERYMA; COSTA, LEONARDO; CHACON-MIKAHIL, MARA P. T.; TEIXEIRA, ANA M.; FARIAS, ALESSANDRO S.; CAVAGLIERI, CLAUDIA R.. Obesity Increases Gene Expression of Markers Associated With Immunosenescence in Obese Middle-Aged Individuals. FRONTIERS IN IMMUNOLOGY, v. 12, p. 9-pg., 2022-01-05. (17/06643-1, 16/08751-3)