Abstract
The CRISPR technology opened new perspectives for genetically reprogramming living organisms. In particular, CRISPR has greatly impacted the molecular genetics of the yeast Saccharomyces cerevisiae, a microbial workhorse for biotechnological applications. Recently, we proposed a new CRISPR approach (EasyGuide) based on the efficient recombination of gRNAs occurring in vivo in S. cerevisia…