Resumo
The aim of this project is to sequence in the next 18 months 120,000 EST clones generated by the ORESTES low-stringency RT-PCR amplification technique (Dias-Neto et al., PNAS USA. 97: 3491-3496, 2000) using S. mansoni mRNA. We expect to generate 40 to 50 million bases of S. mansoni cDNA, which might represent an estimated average 1 to 1.5 fold coverage of the parasite transcriptome. Diffe…