| Texto completo | |
| Autor(es): |
Cortella, Lucas R. X.
;
Cestari, I. A.
;
S, M.
;
Mazzetto, M.
;
Lasagni, A. F.
;
Cestari, Ismar N.
;
Bastos-Filho, TF
;
Caldeira, EMD
;
Frizera-Neto, A
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | XXVII BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2020; v. N/A, p. 8-pg., 2022-01-01. |
| Resumo | |
The use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) to replace myocardial tissue after an infarct holds great promises. However, hiPSC-CM are phenotypically immature when compared to cells in the adult heart, hampering their clinical application. We aimed to develop and test a surface structuring technique that would improve hiPSC-CM structural maturation. Laser ablation was used to fabricate a micron-pattern on polyurethane surface and evaluated cell morphology, orientation and F-actin assemblage to detect phenotypic changes in response to the microtopography. This topography positively influenced cell morphology regarding to spreading area and elongation, and hiPSC-CM orientation, improving their structural maturation. The methodology thus presented has relatively low cost and is easily scalable, making it relevant for high-throughput applications such as drug screening for the pharma industry. (AU) | |
| Processo FAPESP: | 12/50283-6 - Suporte circulatório em pacientes pediátricos: dispositivo de assistência ventricular e oxigenação extracorpórea |
| Beneficiário: | Idágene Aparecida Cestari |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |