Immunological Effects of Dimethyldioctadecylammonium Bromide and Saponin as Adjuvants for Outer Membrane Vesicles from Neisseria meningitidis

Autor(es)

Correa, Victor Araujo ; Portilho, Amanda Izeli ; De Gaspari, Elizabeth

• Tipo de documento: Artigo Científico
• Fonte: DISEASES; v. 10, n. 3, p. 17-pg., 2022-09-01.

Resumo

The meningococcal disease is a global health threat, but is preventable through vaccination. Adjuvants improve meningococcal vaccines and are able to trigger different aspects of the immune response. The present work evaluated the immune response of mice against Neisseria meningitidis outer membrane vesicles (OMV) complexed with the adjuvants aluminium hydroxide (AH), via subcutaneous route; and dimethyldioctadecylammonium bromide (DDA) or Saponin (Sap), via intranasal/subcutaneous routes. ELISA demonstrated that all adjuvants increased IgG titers after the booster dose, remaining elevated for 18 months. Additionally, adjuvants increased the avidity of the antibodies and the bactericidal titer: OMVs alone were bactericidal until 1:4 dilution but, when adjuvanted by Alum, DDA or Sap, it increased to 1/32. DDA and Sap increased all IgG isotypes, while AH improved IgG1 and IgG2a levels. Thus, Sap led to the recognition of more proteins in Immunoblot, followed by DDA and AH. Sap and AH induced higher IL-4 and IL-17 release, respectively. The use of adjuvants improved both cellular and humoral immune response, however, each adjuvant contributed to particular parameters. This demonstrates the importance of studying different adjuvant options and their suitability to stimulate different immune mechanisms, modulating the immune response. (AU)

• Processo FAPESP: 12/15568-0 - Estudo da imunogenicidade de antígenos de membrana externa de Neisseria meningitidis, e interação com lípides catiônicos: avaliação de diferentes vias de imunização em diferentes linhagens de camundongos
• Beneficiário: Elizabeth Natal de Gaspari
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/02042-9 - Avaliação dos perfis de resposta Th1 e Th2 em camundongos imunizados com lípides catiônicos e aniônicos complexados a vesículas de membrana externa de Neisseria meningitis b
• Beneficiário: Victor Araujo Correa
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/04202-0 - Estudo da imunogenicidade de antígenos de membrana externa de Neisseria meningitidis e interação com lípides catiônicos: avaliação de novo adjuvante para Neisseria meningitidis em diferentes linhagens de camundongos idosos
• Beneficiário: Elizabeth Natal de Gaspari
• Modalidade de apoio: Auxílio à Pesquisa - Regular