| Texto completo | |
| Autor(es): Mostrar menos - |
Arana, M. C.
;
Lahuerta, R. D.
;
Cortella, L. R. X.
;
Mazzetto, M.
;
Soldera, M.
;
Lasagni, A. F.
;
Cestari, I. N.
;
Cestari, I. A.
;
Bastos-Filho, TF
;
Caldeira, EMD
;
Frizera-Neto, A
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | XXVII BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2020; v. N/A, p. 5-pg., 2022-01-01. |
| Resumo | |
In this work, we developed and tested an experimental apparatus to evaluate calcium fluctuations in cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CM). The set-up is composed of a signal module for registration and analysis of the signals and a perfusion chamber. This chamber allows the maintenance of the cells, control of perfusion and temperature, and electric stimulation. The signal module consists of a CCD camera attached to a fluorescence microscope with the appropriate software and hardware for eliciting and recording fluorescent signals originating from hiPSC-CM intracellular Ca+2 concentration changes, under electrical stimulation. We employed this system for analysis of calcium fluctuation from hiPSC-CM cultivated on micro textured polyethylene terephthalate surfaces. (AU) | |
| Processo FAPESP: | 12/50283-6 - Suporte circulatório em pacientes pediátricos: dispositivo de assistência ventricular e oxigenação extracorpórea |
| Beneficiário: | Idágene Aparecida Cestari |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |