Enhanced photocatalysis on graphitic carbon nitride sensitized with gold nanoparticles for photoelectrochemical immunosensors

Autor(es)

Neto, Jose L. Bott ; Martins, Thiago S. ; Machado, Sergio A. S. ; Oliveira Jr, Osvaldo N.

• Tipo de documento: Artigo Científico
• Fonte: Applied Surface Science; v. 606, p. 6-pg., 2022-12-30.

Resumo

Photoelectrochemical (PEC) sensors are promising for monitoring the environment and in clinical diagnosis, but their point-of-use application requires portable systems and materials with high photocatalytic activity. In this work, we extend the use of a recent miniaturized PEC system to detect the breast cancer biomarker carbohydrate antigen 15-3 (CA15-3) with the enhanced catalytic activity of graphitic carbon nitride (CN) decorated with gold nanoparticles (Au NPs). The performance of the immunosensor was highly dependent on the size of the nano -particles and on the film architecture. Optimized sensing of CA15-3 was reached with electrodeposited 13.1 nm Au NPs linked to a long-chain mercapto (11-mercaptoundecanoic acid), which allowed for an effective adsorption of antibodies. CA15-3 could be detected from 0.1 fg mL-1 to 10 ng mL-1, with detection limit of 0.04 fg mL-1. As a demonstration of the viability of the PEC immunosensor, the biomarker CA15-3 was detected in human serum with recovery values between 98 and 109%. (AU)

• Processo FAPESP: 19/13514-9 - Sensores eletroquímicos com matriz contendo pontos quânticos ou nanofitas de grafeno para detecção de biomarcadores
• Beneficiário: Jose Luiz Bott Neto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/01777-5 - Confecção de dispositivos eletroquímicos descartáveis compostos por nanoesferas ocas de carbono e nanofios de carbono decorados com nanopartículas de prata para a quantificação de interferentes endócrinos em amostras de água de abastecimento
• Beneficiário: Sergio Antonio Spinola Machado
• Modalidade de apoio: Auxílio à Pesquisa - Regular