Silencing of a Pectin Acetylesterase (PAE) Gene Highly Expressed in Tobacco Pistils Negatively Affects Pollen Tube Growth

Autor(es)

Lubini, Greice ; Ferreira, Pedro Boscariol ; Quiapim, Andrea Carla ; Brito, Michael Santos ; Cossalter, Viviane ; Pranchevicius, Maria Cristina S. ; Goldman, Maria Helena S.

• Tipo de documento: Artigo Científico
• Fonte: PLANTS-BASEL; v. 12, n. 2, p. 16-pg., 2023-01-01.

Resumo

Successful plant reproduction and fruit formation depend on adequate pollen and pistil development, and pollen-pistil interactions. In Nicotiana tabacum, pollen tubes grow through the intercellular spaces of pistil-specialized tissues, stigmatic secretory zone, and stylar transmitting tissue (STT). These intercellular spaces are supposed to be formed by the modulation of cell wall pectin esterification. Previously we have identified a gene preferentially expressed in pistils encoding a putative pectin acetylesterase (PAE), named NtPAE1. Here, we characterized the NtPAE1 gene and performed genome-wide and phylogenetic analyses of PAEs. We identified 30 PAE sequences in the N. tabacum genome, distributed in four clades. The expression of NtPAE1 was assessed by RT-qPCR and in situ hybridization. We confirmed NtPAE1 preferential expression in stigmas/styles and ovaries and demonstrated its high expression in the STT. Structural predictions and comparisons between NtPAE1 and functional enzymes validated its identity as a PAE. Transgenic plants were produced, overexpressing and silencing the NtPAE1 gene. Overexpressed plants displayed smaller flowers while silencing plants exhibited collapsed pollen grains, which hardly germinate. NtPAE1 silencing plants do not produce fruits, due to impaired pollen tube growth in their STTs. Thus, NtPAE1 is an essential enzyme regulating pectin modifications in flowers and, ultimately, in plant reproduction. (AU)

• Processo FAPESP: 19/24774-1 - A importância do gene SCI1 no meristema floral, sua regulação transcricional e conservação evolutiva
• Beneficiário: Maria Helena de Souza Goldman
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/50213-2 - Estudo do desenvolvimento e função do pistilo de Nicotiana tabacum (l.): caracterização de genes expressos no estigma/estilete e das interações proteína-proteína
• Beneficiário: Maria Helena de Souza Goldman
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/16845-4 - Produção de ArtinM recombinante nas formas oligomérica e monomérica: estudo estrutural e análise comparativa de atividades.
• Beneficiário: Douglas Dalle Luche
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/54431-9 - Caracterizacao de genes preferencialmente expressos no estigma/ estilete de nicotiana tabacum, identificados por macroarray e ssh.
• Beneficiário: Maria Helena de Souza Goldman
• Modalidade de apoio: Auxílio à Pesquisa - Regular