Co-expression network of heat-response transcripts: A glimpse into how splicing factors impact rice basal thermotolerance

Autor(es)

Boulanger, Hadrien Georges ; Guo, Wenbin ; Rebocho Monteiro, Lucca de Filipe ; Calixto, Cristiane Paula Gomes

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MOLECULAR BIOSCIENCES; v. 10, p. 10-pg., 2023-02-02.

Resumo

To identify novel solutions to improve rice yield under rising temperatures, molecular components of thermotolerance must be better understood. Alternative splicing (AS) is a major post-transcriptional mechanism impacting plant tolerance against stresses, including heat stress (HS). AS is largely regulated by splicing factors (SFs) and recent studies have shown their involvement in temperature response. However, little is known about the splicing networks between SFs and AS transcripts in the HS response. To expand this knowledge, we constructed a co-expression network based on a publicly available RNA-seq dataset that explored rice basal thermotolerance over a time-course. Our analyses suggest that the HS-dependent control of the abundance of specific transcripts coding for SFs might explain the widespread, coordinated, complex, and delicate AS regulation of critical genes during a plant's inherent response to extreme temperatures. AS changes in these critical genes might affect many aspects of plant biology, from organellar functions to cell death, providing relevant regulatory candidates for future functional studies of basal thermotolerance. (AU)

• Processo FAPESP: 19/13158-8 - Análise global do transcriptoma de arroz relacionado à memória de temperatura e respostas ao calor
• Beneficiário: Cristiane Paula Gomes Calixto
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 22/02633-0 - Análise funcional in silico e construção de plasmídeo para nocaute CRISPR/Cas9-mediado do gene responsivo a estresse térmico Telomere Repeat-Binding Factor 1 em arroz (Oryza sativa L.)
• Beneficiário: Lucca de Filipe Rebocho Monteiro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica