| Texto completo | |
| Autor(es): |
Alvarez, Rafael Herrera
;
Barros do Amaral, Jackson
;
Ladeira Pires, Rita Maria
;
Martinez, Antonio Campanha
;
Oba, Eunice
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Boletim da Indústria Animal; v. 65, n. 3, p. 6-pg., 2008-01-01. |
| Resumo | |
This study evaluated pregnancy rate, gestation length, calving rate, and also sex proportion and birth weight of calves derived from in vitro reconstructed bovine demi-embryos. Recovered embryos (grade I morulae) from superovulated cows were bisected with a steel blade and transferred to recipient heifers either immediately (group 1, n= 38) or after a 24h period in culture medium (EARLE + 10% FCS) at 38.5 degrees C and 5% CO 2 (group 2, n= 34) when showed a reconstituted appearance. Intact, uncultured embryos immediately transferred to recipients after collection (group 3, n= 36) were used as control. Data were analyzed by ANOVA and Chi-square test. In vitro cultured demi-embryos that developed from morulae to blastocyst (78%, 44/ 56) were considered viable. Pregnancy rate at day 45 was 47.4%, 52.9%, and 52.8% for groups 1, 2, and 3 respectively (P > 0.05). Calving rate reached 26.3% in group 1. 29.4% in group 2, and 30.6% in group 3 (P> 0.05). No difference was found regarding pregnancy length (280.8 +/- 1.8, 285.5 +/- 1.7 and 285.3 +/- 1.7 days), male proportion (60%, 50% and 54.5%) and birth weight of calves (38 +/- 4.2, 32.4 +/- 2.5 and 36.2 +/- 2.6 kg) among groups 1, 2 and 3, respectively. It was concluded that even though in vitro reconstructed demi-embryos transfer can be of some utility to select the viability of demi-embryos, this procedure is not able to improve pregnancy rates since it does not differ from uncultured demi-embryos. (AU) | |
| Processo FAPESP: | 01/10514-4 - Taxa de prenhez apos transferencia de embrioes bovinos bipartidos e reconstituidos in vitro. |
| Beneficiário: | Rafael Herrera Alvarez |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |