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Maximal lactate steady state for aerobic evaluation of swimming mice

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Autor(es):
Gobatto, Claudio Alexandre ; Manchado-Gobatto, Fulvia de Barros ; Carneiro, Ligia Giuzio ; de Araujo, Gustavo Gomes ; Masselli dos Reis, Ivan Gustavo
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: COMPARATIVE EXERCISE PHYSIOLOGY; v. 6, n. 3, p. 5-pg., 2009-08-01.
Resumo

The maximal lactate steady state (MLSS) has been considered the gold standard method to determine aerobic/anaerobic metabolism transition during continuous exercise executed by human beings and rats. Therefore, the aim of the present study was to determine MLSS for aerobic evaluation in swimming mice. Twenty-five adult male mice (90-day-old animals) were adapted to the deep aquatic environment at the temperature of 31 +/- 1 degrees C. The mice were submitted to five continuous exercise loads of 3, 4, 5, 6 and 7% of the body weight (bw) tied to the back, executed with 25-min duration and 48-h intervals between them. Blood samples were collected from the tail during swimming exercise (rest, 5, 10, 15, 20 and 25 min) for blood lactate concentration (LAC) determinations. The individual MLSS was considered as the highest intensity in which the increase on the LAC was equal to or below 1mmol l (-1) from the 10th to the 25th minute of exercise. The results showed that 36% of the swimming mice presented MLSS at 4% bw, 20% at 3% bw and 6% bw, 16% at 5% bw and 8% at 7% bw. The LAC at the MLSS was 5.78 +/- 0.29 mmol l (-1) (4.40- 6.67 mmol l (-1)). These results indicate that the MLSS of mice swimming with additional weight for the final 15 of 25 min of exercise could be determined. (AU)

Processo FAPESP: 06/58411-2 - Efeitos de quatro modelos de treinamento experimental de natação sobre biomarcadores de adaptação e capacidades aeróbia e anaeróbia em ratos Wistar
Beneficiário:Gustavo Gomes de Araujo
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 06/61189-0 - Padronizacao de um metodo para determinacao da densidade corporal e sobrecarga no modelo de natacao em ratos wistar.
Beneficiário:Ivan Gustavo Masselli dos Reis
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica