Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples

Autor(es)

Maia Faria, Joice Lara ; Dagnone, Ana Silvia ; Munhoz, Thiago Demarchi ; Joao, Carolina Franchi ; Biscola Pereira, Wanderson Adriano ; Machado, Rosangela Zacarias ; Tinucci-Costa, Mirela

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 19, n. 2, p. 5-pg., 2010-04-01.

Resumo

The aim of this study was to compare the detection of Ehrlichta cams morulae and DNA by nPCR in whole blood and spleen aspiration The sample Included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis Morulae detection showed that in 35 of the dogs studied, 17 had morulae in spleen tissue, and two in buffy coat smears E cants DNA was detected in 29/40 blood samples We verified that morulae detection is more efficient in cytological preparations from spleen aspiration On the other hand, nPCR on spleen and blood samples were equally efficient for disease diagnosis (AU)

• Processo FAPESP: 04/04383-2 - Estudo comparativo de deteccao de dna de ehrlichia canis em sangue total e em aspirado de baco pelo nested pcr de caes naturalmente infectados.
• Beneficiário: Mirela Tinucci Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular