Bioluminescence of Beetle Luciferases with 6 '-Amino-D-luciferin Analogues Reveals Excited Keto-oxyluciferin as the Emitter and Phenolate/Luciferin Binding Site Interactions Modulate Bioluminescence Colors

Autor(es)

Viviani, Vadim R. ; Rodrigues Neves, Deimison ; Trabuco Amaral, Danilo ; Prado, Rogilene A. ; Matsuhashi, Takuto ; Hirano, Takashi

• Tipo de documento: Artigo Científico
• Fonte: BIOCHEMISTRY; v. 53, n. 32, p. 13-pg., 2014-08-19.

Resumo

Beetle luciferases produce different bioluminescence colors from green to red using the same D-luciferin substrate. Despite many studies of the mechanisms and structural determinants of bioluminescence colors with firefly luciferases, the identity of the emitters and the specific active site interactions responsible for bioluminescence color modulation remain elusive. To address these questions, we analyzed the bioluminescence spectra with 6'-amino-D-luciferin (aminoluciferin) and its 5,5-dimethyl analogue using a set of recombinant beetle luciferases that naturally elicit different colors and different pH sensitivities (pH-sensitive, Amydetes vivianii lambda(max) = 538 nm, Macrolampis sp(2) lambda(max) = 564 nm; pH-insensitive, Phrixotrix hirtus lambda(max) = 623 nm, Phrixotrix vivianii lambda(max) = 546 nm, and Pyrearinus termitilluminans lambda(max) = 534 nm), a luciferase-like enzyme (Tenebrionidae, Zophobas mono lambda(max) = 613 nm), and mutants of C311 (S314). The green-yellow-emitting luciferases display red-shifted bioluminescence spectra with aminoluciferin in relation to those with D-luciferin, whereas the red-emitting luciferases displayed blue-shifted spectra. Bioluminescence spectra with 5,5-dimethylaminoluciferin, in which enolization is blocked, were almost identical to those of aminoluciferin. Fluorescence probing using 2-(4-toluidino)naphthalene-6-sulfonate and inference with aminoluciferin confirm that the luciferin binding site of the red-shifted luciferases is more polar than in the case of the green-yellow-emitting luciferases. Altogether, the results show that the keto form of excited oxyluciferin is the emitter in beetle bioluminescence and that bioluminescence colors are essentially modulated by interactions of the 6'-hydroxy group of oxyluciferin and basic moieties under the influence of the microenvironment polarity of the active site: a strong interaction between a base moiety and oxyluciferin phenol in a hydrophobic microenvironment promotes green-yellow emission, whereas a more polar environment weakens such interaction promoting red shifts. In pH-sensitive luciferases, a pH-mediated switch from a closed hydrophobic conformation to a more open polar conformation promotes the typical red shift. (AU)

• Processo FAPESP: 11/23961-0 - Luciferases pH-sensitivas: origem estrutural da sensibilidade ao pH, e viabilidade de emprego em biossensores celulares de pH e cátions de metais pesados
• Beneficiário: Vadim Viviani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/04857-0 - Origem e evolução estrutural da atividade oxigenase/luciferásica em AMP/CoA-ligases e luciferases de besouros
• Beneficiário: Vadim Viviani
• Modalidade de apoio: Auxílio à Pesquisa - Regular