Identification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs

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Viol, Milena Arauz ; Guerrero, Felix D. ; Miranda de Oliveira, Bruno Cesar ; Costa de Aquino, Monally Conceicao ; Loiola, Saulo Hudson ; de Melo, Guilherme Dias ; de Souza Gomes, Aparecida Helena ; Kanamura, Cristina Takami ; Garcia, Marcos Valerio ; Andreotti, Renato ; Felix de Lima, Valeria Marcal ; Saraiva Bresciani, Katia Denise

• Tipo de documento: Artigo Científico
• Fonte: Parasitology Research; v. 115, n. 9, p. 6-pg., 2016-09-01.

Resumo

Sand flies are recognized as the major vector of canine visceral leishmaniasis. However, in some areas of Brazil where sand flies do not occur, this disease is found in humans and dogs. There has been speculation that ticks might play a role in transmission of canine visceral leishmaniasis and the DNA of Leishmania spp. has been reported in whole ticks. We investigated the presence of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus ticks collected from tick-infested dogs in two cities of Brazil. We used 66 dogs that tested positive and 33 that tested negative for Leishmania spp. according to direct cytological examination assays. Ten ticks were collected from each dog and dissected to collect the intestines, ovaries, and salivary glands for immunohistochemistry (IHC) and diagnostic real-time polymerase chain reaction (RT-PCR). IHC results showed Leishmania spp. in 98, 14, and 8 % of the intestines, ovaries, and salivary glands, respectively. Real-time PCR showed that 89, 41, and 33 % of the tick intestine, ovary, and salivary glands, respectively, were positive for Leishmania spp. The verification of promastigotes of Leishmania spp. by two independent techniques in ticks collected from these urban region dogs showed that there is need for clarification of the role of ticks in the transmission of canine visceral leishmaniasis in Brazil. (AU)

• Processo FAPESP: 12/00266-8 - Investigação epidemiológica, imunológica e molecular de Leishmania (Leishmania) chagasi em carrapatos Rhipicephalus sanguineus em cães dos estados de São Paulo e Mato Grosso do Sul
• Beneficiário: Milena Araúz Viol
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/51113-4 - Investigacao epidemiologica e molecular de leishmania (leishmania) chagasi em carrapatos rhipicephalus sanguineus em caes dos estados de sao paulo e mato grosso do sul.
• Beneficiário: Katia Denise Saraiva Bresciani
• Modalidade de apoio: Auxílio à Pesquisa - Regular