| Texto completo | |
| Autor(es): |
Sartori, Roger
;
Leme, Jaci
;
Caricati, Celso Pereira
;
Tonso, Aldo
;
Fernandez Nunez, Eutimio Gustavo
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Journal of Chemical Engineering; v. 35, n. 2, p. 18-pg., 2018-04-01. |
| Resumo | |
Baby Hamster Kidney cells (BHK-21) are commonly used in research and the biopharmaceutical industry. This work aimed to model the kinetic performance in batch operation mode of BHK-21 cells cultured in two stirred tank configurations using different dissolved oxygen concentrations and pH control strategies. Viable and dead cell concentrations, as well as glucose, glutamine, lactate and ammonium concentrations, were monitored. Statistical multiple linear regression, logistic equation and multiplicative Monod kinetic models were fitted. Statistical models for viable cells concentration as a function of nutrient and metabolite concentrations were significant (R-2 > 0.91). Logistic model parameters: intrinsic growth rate, cell density level in the medium and time for reaching maximum cell concentrations were within 0.061-0.083 h(-1), 1.85-5.39 x 10(9) cell L-1 and 52-90 h ranges, respectively. A Monod-type model was satisfactorily fitted to the experimental data. Relative errors were lower than 10% for six monitored state variables in most of the assessed experimental conditions. The three models developed in this work can be used in bioprocesses involving BHK-21 with good fitting. (AU) | |
| Processo FAPESP: | 10/52521-6 - Producao de glicoproteinas da raiva atraves do cultivo de celulas bhk-21 em biorreatores usando sistema de expressao semliki forest virus (sfv). |
| Beneficiário: | Eutimio Gustavo Fernández Núñez |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |