Enabling butanol production from crude sugarcane bagasse hemicellulose hydrolysate by batch-feeding it into molasses fermentation

Autor(es)

Chacon, Suranny Jimenez ; Matias, Gabriela ; dos Santos Vieira, Carla Ferreira ; Ezeji, Thaddeus Chukwuemeka ; Maciel Filho, Rubens ; Mariano, Adriano Pinto

• Tipo de documento: Artigo Científico
• Fonte: INDUSTRIAL CROPS AND PRODUCTS; v. 155, p. 6-pg., 2020-11-01.

Resumo

Butanol is a chemical and advanced biofuel whose fermentative production from hemicellulose hydrolysates is hindered by lignocellulose-derived microbial inhibitory compounds. In this work, Clostridium saccharoperbutylacetonicum DSM 14923 was not able to ferment a sugarcane hemicellulose hydrolysate supplemented with either laboratory-grade nutrients or sugarcane molasses. To circumvent this problem, the crude hemicellulose hydrolysate was fed, after 24 h, to a molasses fermentation containing 45 g/L total reducing sugars. Without the need of supplementing nutrients, the culture was able to co-ferment sucrose, glucose, fructose, and xylose (conversion of sucrose and xylose were 81 and 90 %, respectively) in a fermentation containing a diluted molasses-to-hydrolysate volume ratio of 3:1. Butanol yield (0.31 g/g) was remarkable, and butanol titer was 10.0 g/L after 72 h of fermentation. Therefore, this research demonstrated that sugarcane molasses a byproduct of the sugar industry rich in sucrose and nutrients can - be an efficient feedstock for enabling the production of butanol from sugarcane bagasse hemicellulose hydrolysate. (AU)

• Processo FAPESP: 18/25925-0 - Produção de n-butanol a partir da fermentação de hidrolisado hemicelulósico do bagaço da cana-de-açúcar com imobilização celular
• Beneficiário: Gabriela Matias
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/20630-4 - Desenvolvimento integrado de biorrefinaria e planta de bioetanol de cana-de-açúcar com emissão zero de CO2: rotas para converter recursos renováveis em bioprodutos e bioeletricidade
• Beneficiário: Rubens Maciel Filho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/23983-3 - Rotas para produção de pneus a partir da cana-de-açúcar: conversão eficiente de açúcares em precursores de 1,3-butadieno e avaliação técnico-econômica ambiental
• Beneficiário: Adriano Pinto Mariano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular