| Texto completo | |
| Autor(es): |
Sepulchro, Ana Gabriela Veiga
;
Vacilotto, Milena Moreira
;
Dias, Lucas D.
;
Pellegrini, Vanessa O. A.
;
Velasco, Josman
;
Inada, Natalia M.
;
Segato, Fernando
;
Polikarpov, Igor
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CHEMBIOCHEM; v. N/A, p. 14-pg., 2024-11-11. |
| Resumo | |
The use of light as abundant, renewable, and clean energy source to boost lytic polysaccharide monooxygenase (LPMO) reactions represents an exciting and yet under-explored opportunity. Herein we demonstrated that photosensitizers, commonly used in photodynamic therapy, which act through the photocatalytic Type I mechanism can drive the oxidation of PASC by LPMOs, whereas Type II photosensitizers are not capable of promoting the LPMO activity. We analyzed Type I and Type II photosensitizers (methylene blue and tetraiodide salt of meso-tetrakis-(4-N-methylpyridyl) porphyrin, respectively) and demonstrated that, even without an addition of external reductant, Type I was capable of boosting Thermothelomyces thermophila MtLPMO9A activity in the presence of light. We also evaluated the photobiosystem in the presence and/or absence of molecular oxygen (O2) and hydrogen peroxide (H2O2), and investigated the role of superoxide radical in the methylene blue fueled reactions. Furthermore, we demonstrated that sodium bisulfite (NaHSO3), a chemical scavenger of H2O2, acts by safeguarding the enzyme from oxidative damage caused by accumulation of H2O2 early in photosensitizer-driven LPMO reactions. Finally, the results of the present work demonstrated that light-driven LPMO reactions mediated photodynamic therapy (PDT) Type I photosensitizers, which also includes molecules such as curcumin and riboflavin, is a general phenomenon. (AU) | |
| Processo FAPESP: | 21/08780-1 - DEGRADAÇÃO ENZIMÁTICA DE EXOPOLISSACHARÍDEOS DE BIOFILMES MICROBIANOS: Abordagens de Biofísica Estrutural, Biotecnologia Molecular e Bioquímica Sintética em estudos de CAZymes em busca de novas ferramentas enzimáticas para tratamentos antimicrobianos |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 24/00533-3 - Biorrefino de hemiceluloses e lignina do bagaço de cana-de-açúcar |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/13569-8 - Estudo dos Mecanismos de Ação em Terapia Fotodinâmica: Do Fotossensibilizador à Aplicação Prática |
| Beneficiário: | Lucas Danilo Dias |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica |
| Beneficiário: | Vanderlei Salvador Bagnato |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida |
| Beneficiário: | Vanderlei Salvador Bagnato |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |