| Texto completo | |
| Autor(es): |
Harb, Samarah V.
;
Kolanthai, Elayaraja
;
Backes, Eduardo H.
;
Beatrice, Cesar A. G.
;
Pinto, Leonardo A.
;
Nunes, Ana Carolina C.
;
Selistre-de-Araujo, Heloisa S.
;
Costa, Lidiane C.
;
Seal, Sudipta
;
Pessan, Luiz Antonio
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | TISSUE ENGINEERING AND REGENERATIVE MEDICINE; v. 21, n. 2, p. 20-pg., 2023-10-19. |
| Resumo | |
Background:Poly (lactic acid) (PLA) is a biodegradable polyester that has been exploited for a variety of biomedical applications, including tissue engineering. The incorporation of beta-tricalcium phosphate (TCP) into PLA has imparted bioactivity to the polymeric matrix. Methods:We have modified a 90%PLA-10%TCP composite with SiO2 and MgO (1, 5 and 10 wt%), separately, to further enhance the material bioactivity. Filaments were prepared by extrusion, and scaffolds were fabricated using 3D printing technology associated with fused filament fabrication. Results:The PLA-TCP-SiO2 composites presented similar structural, thermal, and rheological properties to control PLA and PLA-TCP. In contrast, the PLA-TCP-MgO composites displayed absence of crystallinity, lower polymeric molecular weight, accelerated degradation ratio, and decreased viscosity within the 3D printing shear rate range. SiO2 and MgO particles were homogeneously dispersed within the PLA and their incorporation increased the roughness and protein adsorption of the scaffold, compared to a PLA-TCP scaffold. This favorable surface modification promoted cell proliferation, suggesting that SiO2 and MgO may have potential for enhancing the bio-integration of scaffolds in tissue engineering applications. However, high loads of MgO accelerated the polymeric degradation, leading to an acid environment that imparted the composite biocompatibility. The presence of SiO2 stimulated mesenchymal stem cells differentiation towards osteoblast; enhancing extracellular matrix mineralization, alkaline phosphatase (ALP) activity, and bone-related genes expression. Conclusion:The PLA-10%TCP-10%SiO2 composite presented the most promising results, especially for bone tissue regeneration, due to its intense osteogenic behavior. PLA-10%TCP-10%SiO2 could be used as an alternative implant for bone tissue engineering application. (AU) | |
| Processo FAPESP: | 18/26060-3 - Scaffolds bioativos e bactericidas para regeneração óssea via impressão 3D |
| Beneficiário: | Samarah Vargas Harb |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/09609-9 - Desenvolvimento de scaffolds bioinspirados de PLA/cargas cerâmicas bioativas via impressão 3D |
| Beneficiário: | Luiz Antonio Pessan |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/27415-2 - Scaffolds bioativos com modificação superficial por plasma |
| Beneficiário: | Luiz Antonio Pessan |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/11366-7 - Desenvolvimento de scaffolds bioinspirados de PLA/biovidros via impressão 3D |
| Beneficiário: | Eduardo Henrique Backes |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 21/11538-8 - Bioatividade e ação bactericida de scaffold constiuídos de PLA-TCP modificado com SiO2, ZnO e CeO2 e obtidos por impressão 3D aplicados para engenharia de tecido ósseo |
| Beneficiário: | Samarah Vargas Harb |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |