A new adsorptive 3D-printed sampling device for simultaneous determination of 63 urinary organic acids by LC-MS/MS

Autor(es)

Bocato, Mariana Zuccherato ; Quero, Reverson Fernandes ; Weil, Anthony Alexandre ; Cesila, Cibele Aparecida ; Adeyemi, Joseph Adewuyi ; Barbosa Jr, Fernando

• Tipo de documento: Artigo Científico
• Fonte: Analytica Chimica Acta; v. 1288, p. 13-pg., 2024-01-01.

Resumo

Background: The detection and quantification of urinary metabolites play an important role in disease diagnosis. In most cases, urinary analyses are done with liquid urine samples, which must be quickly transported to the laboratory to avoid metabolites degradation that is associated with temperature fluctuations. Consequently, dried sampling devices have emerged to minimize analyte degradation. However, most commercial dried sampling devices are expensive, aggregate low volumes, and need better analytical sensitivity. Therefore, a new dry urine sampling device that is inexpensive, suitable for domestic sampling operation, and efficient for quantifying metabolites without requiring high-resolution instruments is proposed in the present study. Results: The newly designed dry urine sampling device was produced by 3D printing that efficiently determines 63 urinary organic acids using liquid chromatography coupled with mass spectrometry (LC-MS/MS). The system's efficiency was demonstrated with analytical figures of merit, such as precision, accuracy, and stability of analytes after the sampling and storing of ordinary urine samples. The limits of quantification ranged from 0.01 to 0.42 ng mL-1. Precision and accuracy tests showed relative standard deviations of less than 15 %. The urine stability in the sampling device was high within seven days without any significant degradation of the metabolites. The method was applied to the analysis of 10 human urine samples and compared to a conventional method without the use of the sampling device. The results showed no statistically significant differences, demonstrating the method's efficiency. Significance: The proposed 3-D printing device was developed with fast, low-cost manufacturing features and can be manufactured with different volumetric capacities, adaptable to the needs of each user. Furthermore, it is innovative because this is the first sampling device that is effective for the simultaneous storage and preservation of several important urinary metabolites. Thus, it is anticipated that its application would contribute significantly to the identification of metabolic disorders. (AU)

• Processo FAPESP: 22/10546-0 - Desenvolvimento de tecnologias rápidas e eficientes para diagnóstico de biomarcadores críticos relacionados à processos bioquímicos, nutrição e meio ambiennutrição
• Beneficiário: Mariana Zuccherato Bocato Kimachi
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE
• Processo FAPESP: 20/05839-2 - Desenvolvimento de tecnologias rápidas e eficientes para diagnóstico de biomarcadores críticos relacionados a processos bioquímicos, nutrição e meio ambiente visando um estilo de vida saudável
• Beneficiário: Anthony Alexandre Weil
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE
• Processo FAPESP: 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 23/05017-0 - Desenvolvimento de tecnologias rápidas e eficientes para diagnóstico de biomarcadores críticos relacionados à processos bioquímicos, nutrição e meio ambiente
• Beneficiário: Mariana Zuccherato Bocato Kimachi
• Modalidade de apoio: Bolsas no Brasil - Pesquisa Inovativa em Pequenas Empresas - PIPE