| Texto completo | |
| Autor(es): |
Santos-Oliveira, Pedro Henrique
;
Machado, Nathalia Fernandes Gonsalves
;
de Oliveira, Rafael David
;
Velasco-Yepez, Eric Andre
;
da Silva, Sonia Regina
;
Rocha, Rafael Costa Santos
;
Blank, Lars Mathias
;
Silva, Luiziana Ferreira
;
Le Rouxc, Galo Antonio Carrillo
;
Gomez, Jose Gregorio Cabrera
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Bioresource Technology; v. 391, p. 10-pg., 2023-11-08. |
| Resumo | |
Pseudomonas sp. LFM046 produces polyhydroxyalkanoates of medium-chain length. When carbohydrates are used, only even-chain lenght monomers (3HAeven) are generated. Propionate, when used as a co-substrate, enabled the synthesis of odd-chain length monomers, albeit with poor yields (Y3HAodd/prop.) of approx. 10 %. A mini-Tn5 mutant (LFM693) was generated by interrupting the prpB gene, which encodes the 2-methylisocitrate lyase responsible for one step in propionate oxidation via 2-methylcitrate cycle. At low propionate concentrations of 0.1-0.3 g.L-1, LFM693 showed higher Y3HAodd/prop. In contrast, the propionyl incorporation into the polymer was less efficient at high propionate concentrations. The excretion of 2-methylisocitrate partially explained this reduced efficiency. However, mass balances and 13C isotopomer analysis revealed significant propionate oxidation in LFM693 of 21 % and 46 % for propionate concentrations of 0.5 and 1.0 g.L-1, respectively. Experiments using C1-labeled (13C) propionate revealed an alternative propionate oxidation pathway (alpha-oxidation, beta-oxidation, or 3-methylmalate) whose genes are yet to be revealed. (AU) | |
| Processo FAPESP: | 03/01602-2 - Avaliação do metabolismo de síntese de PHAMCL e controle de sua composição monomérica |
| Beneficiário: | José Gregório Cabrera Gomez |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 13/50357-2 - Programmable balancing of growth and formation of poyhydroxyalkanoates in Escherichia coli |
| Beneficiário: | José Gregório Cabrera Gomez |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |