Antiradical capacity assay for hydrophobic substances using hemin-catalyzed luminol chemiluminescence in cationic micelles

Autor(es)

Santos, Cerize S. ; Lemos, Marcos P. O. ; Betschart, Larissa M. ; Baader, Wilhelm J.

• Tipo de documento: Artigo Científico
• Fonte: Photochemistry and Photobiology; v. 100, n. 6, p. 16-pg., 2024-02-23.

Resumo

Antioxidant substances which can diminish the steady-state concentration of free radicals in vivo are important in the human dietary to diminish the deleterious effects of oxidative stress. As the potential of certain substances as antioxidants is difficult to be verified in vivo, simple chemical in vitro assays which test the potential of substances as antioxidants are of great importance for the screening of new antioxidants. These assays measure the capacity of a substance to suppress free radicals. We describe here an antiradical capacity assay, based on luminol chemiluminescence, in cationic micellar medium, allowing the capacity determination of hydrophobic compounds. The antiradical capacity of antioxidants is determined using the Trolox standard by the measurement of the light emission inhibition area caused by the addition of different antiradical concentrations. The obtained results are compared to the values determined using the scavenging of stable free radicals be the substances and shown to be similar for compounds like uric acid, rutin, and quercetin. However, for vitamin E, the luminol assay results in a considerably higher antiradical capacity than the assay with a stable free radical, which is rationalized by the higher reactivity of the radical generated in the luminol assay and a specific localization of vitamin E in the micellar medium. Hemin-catalyzed luminol chemiluminescence assay in micellar medium allows the determination of antiradical capacity of hydrophobic antioxidants giving rise to higher antiradical capacity valued for vitamin E than formerly determined.image (AU)

• Processo FAPESP: 14/22136-4 - Uso de solventes verdes e suas misturas na otimização de processos químicos
• Beneficiário: Omar Abou El Seoud
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 21/13791-2 - EMU concedido no processo 2014/22136-4: Fluorímetro Steady State Spectrometer, Edinburgh Instruments
• Beneficiário: Omar Abou El Seoud
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 23/06417-2 - Mecanismos da Quimiluminescência Orgânicas
• Beneficiário: Joséf Wilhelm Baader
• Modalidade de apoio: Auxílio à Pesquisa - Regular