| Texto completo | |
| Autor(es): |
da Silva, Isabela Sanches Pompeo
;
Bordini, Ester Alves Ferreira
;
Bronze-Uhle, Erika Soares
;
de Stuani, Vitor
;
Costa, Matheus Castro
;
de Carvalho, Leticia Alves Martins
;
Cassiano, Fernanda Balestrero
;
de Azevedo Silva, Lucas Jose
;
Borges, Ana Flavia Sanches
;
Soares, Diana Gabriela
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Biomedical Materials Research Part A; v. N/A, p. 16-pg., 2024-07-17. |
| Resumo | |
The objective of this study was to create injectable photo-crosslinkable biomaterials, using gelatin methacryloyl (GelMA) hydrogel, combined with a decellularized bone matrix (BMdc) and a deproteinized (BMdp) bovine bone matrix. These were intended to serve as bioactive scaffolds for dentin regeneration. The parameters for GelMA hydrogel fabrication were initially selected, followed by the incorporation of BMdc and BMdp at a 1% (w/v) ratio. Nano-hydroxyapatite (nHA) was also included as a control. A physicochemical characterization was conducted, with FTIR analysis indicating that the mineral phase was complexed with GelMA, and BMdc was chemically bonded to the amide groups of gelatin. The porous structure was preserved post-BMdc incorporation, with bone particles incorporated alongside the pores. Conversely, the mineral phase was situated inside the pore opening, affecting the degree of porosity. The mineral phase did not modify the degradability of GelMA, even under conditions of type I collagenase-mediated enzymatic challenge, allowing hydrogel injection and increased mechanical strength. Subsequently, human dental pulp cells (HDPCs) were seeded onto the hydrogels. The cells remained viable and proliferative, irrespective of the GelMA composition. All mineral phases resulted in a significant increase in alkaline phosphatase activity and mineralized matrix deposition. However, GelMA-BMdc exhibited higher cell expression values, significantly surpassing those of all other formulations. In conclusion, our results showed that GelMA-BMdc produced a porous and stable hydrogel, capable of enhancing odontoblastic differentiation and mineral deposition when in contact with HDPCs, thereby showing potential for dentin regeneration. Representative scheme of hydrogels fabrication. (Upper left) GelMA formulation. (Lower left) BMdc and BMdp fabrication process. (Right) Representation of GelMA-BMdc and GelMA-BMdp hydrogels. image (AU) | |
| Processo FAPESP: | 16/15674-5 - Associação de técnicas de engenharia tecidual para modulação da regeneração de tecidos mineralizados sob inflamação degenerativa: análise em modelos de cultura-3D em biorreator de perfusão e inflamatórios em animais |
| Beneficiário: | Diana Gabriela Soares dos Passos |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 21/09498-8 - Avaliação de hidrogeis bioimpressos contendo matriz óssea bovina descelularizada aplicados para regeneração dentinária |
| Beneficiário: | Isabela Sanches Pompeo da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 22/05888-9 - Desenvolvimento de estratégias para Odontologia Regenerativa baseadas em tecnologias de impressão tri-dimensional |
| Beneficiário: | Diana Gabriela Soares dos Passos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |