| Texto completo | |
| Autor(es): |
Marques, Camila Monteiro Santos
;
Lyra, Lucas Ferreira
;
Pinto, Juliana Guerra
;
Sakane, Kumiko Koibuchi
;
Ferreira-Strixino, Juliana
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 330, p. 12-pg., 2025-01-21. |
| Resumo | |
Acinetobacter baumannii stands out for its antimicrobial resistance and high capacity to cause hospital infections, posing a severe threat to global public health. Thus, there is an urgent need for new therapeutic strategies. This work applied photodynamic therapy (PDT) with curcumin to Acinetobacter baumannii, and bacterial cell viability was assessed. Fourier transform infrared spectroscopy (FT-IR) was used to study changes in biomolecules after PDT application, comparing them with control groups that were only irradiated and only with curcumin photosensitizer. According to the results, there was an increase in the total amount of lipids ( 0.3 %), total proteins ( 0.6 %), and nuclear material ( 8.6 %), with a decrease in carbohydrates ( 3.0 %) after the application of PDT, compared to the control group. In the secondary structure of the proteins, a reduction in alpha-helix ( 3.0 %), disordered structure ( 7.0 %), and turns ( 5.0 %) was observed, with an increase in beta-sheets ( 8.8 %). The analysis of biomolecular changes by FT-IR after the application of PDT can contribute to understanding the mechanisms of action of cell death, enabling therapeutic strategies to improve diagnosis and clinical treatment. (AU) | |
| Processo FAPESP: | 22/10251-0 - Avaliação da terapia fotodinâmica com diferentes compostos para inativação microbiana - Ensaios in vitro e in vivo |
| Beneficiário: | Juliana Guerra Pinto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |