Development of Calvarial-Derived Osteogenic Cells on GDF-5 Coated Nanoporous Titanium Surfaces

Autor(es)

Bueno, Renan B. L. ; Teixeira, Lucas N. ; Pavinatto, Felippe J. ; Maximiano, William M. A. ; Zuardi, Leonardo R. ; Rosa, Adalberto L. ; Oliveira Jr, Osvaldo N. ; Spriano, Silvia ; de Oliveira, Paulo Tambasco

• Tipo de documento: Artigo Científico
• Fonte: METALS; v. 15, n. 2, p. 17-pg., 2025-02-01.

Resumo

This study evaluated the impact of a single variation in the etching time of H2SO4/H2O2-treated titanium (Ti) surfaces on the adsorption of growth and differentiation factor-5 (GDF-5) and their effects on the acquisition of the osteogenic phenotype in vitro. Rat primary calvarial osteogenic cells were grown for up to 14 days on the following Ti surfaces: (1) 30 min: nanotopography obtained with a 1:1 mixture of H2SO4/H2O2 for 30 min (control); (2) 30 min + GDF-5: a 30 min-etched Ti sample adsorbed with recombinant human (rh) GDF-5; (3) 4 h: nanotopography obtained with a 1:1 mixture of H2SO4/H2O2 for 4 h (control); (4) 4 h + GDF-5: a 4 h-etched Ti sample adsorbed with rhGDF-5. The GDF-5 adsorption procedure was carried out on the day before cell plating using 200 ng/mL rhGDF-5 overnight at 4 degrees C. The 30 min- and 4 h-etched Ti samples exhibited a high hydrophilic network of nanopits with a tendency towards larger nanopits for the 4 h group, which corresponded to an enhanced GDF-5 adsorption. For both etching times, coating with GDF-5 resulted in less hydrophilic surfaces that supported (1) a reduction in the proportion of spread cells and an enhanced extracellular osteopontin labeling at early time points of culture, and (2) increased alkaline phosphatase activity preceding an enhanced mineralized matrix formation compared with controls, with a tendency towards higher osteogenic activity for the 4 h + GDF-5 group. In conclusion, the osteogenic potential induced by the GDF-5 coating can be tailored by subtle changes in the nanotopographic characteristics of Ti surfaces. (AU)

• Processo FAPESP: 08/55575-0 - Desenvolvimento do fenotipo osteogenico in vitro sobre diferentes nanotopografias de titanio funcionalizadas com o fator de crescimento gdf-5
• Beneficiário: Renan de Barros e Lima Bueno
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/50342-0 - Funcionalizacao de gdf-5 em superficie nanoestruturada de titanio:estudo in vitro e in vivo.
• Beneficiário: Renan de Barros e Lima Bueno
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/08605-6 - Estudo da expressão de osteopontina em sistemas de coculturas de células osteoblásticas e epiteliais neoplásicas humanas e seus efeitos sobre o fenótipo neoplásico e a ativação osteoclástica
• Beneficiário: Lucas Novaes Teixeira
• Modalidade de apoio: Bolsas no Brasil - Doutorado