UV radiation triggers mycosporine-glutaminol-glucoside biosynthesis in Naganishia friedmannii FBU002, a non-pathogenic yeast

Autor(es)

Martins-Silva, Gabriel ; Barbosa, Renan Santini ; Santos, Ronaldo Silva ; de Carvalho, Ana Carolina Souza Ramos ; Kreusch, Marianne ; Padilla, Adrian Adolfo Alvarez ; Vallim, Marcelo Afonso ; de Medeiros, Livia Soman ; Pascon, Renata Castiglioni

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Microbiology; v. 136, n. 3, p. 11-pg., 2025-03-13.

Resumo

Aims Ultraviolet radiation (UVR) interferes with aspects of life on Earth. It is necessary for the synthesis of important molecules, as vitamin D, but it is harmful to organisms leading to photoaging and skin cancer. Artificial sunscreens prevent these harmful effects, but may be carcinogenic and neurotoxic; also they accumulate in the aquatic ecosystem, harming the environment and leading to coral bleaching. Most artificial sunscreens commercialized are fossil fuel derived and produced by the petrochemical industry. As society turns to bioeconomy, these artificial sunscreens may be substituted by sustainable ones. Algae, cyanobacteria, and fungi produce mycosporines and mycosporine-like aminoacids, which absorb UV radiation and dissipate it as heat. They are a natural source of sunscreen with low or no toxicity and can be produced by biotechnological means; therefore, the aim of this study is to search for mycosporine biosynthesis in yeast from an extreme environment.Methods and results Chromatographic and spectroscopic data analyses demonstrated for the first time an isolate of Naganishia friedmannii, collected from a site with high UVR incidence, is able to produce mycosporine-glutaminol-glucoside (MGG) and its likely diastereoisomer, when exposed to photosynthetically active radiation (PAR)-UVR light. A biosynthetic gene cluster was identified in the N. friedmannii genome and shown to be induced in response to UVR by real-time polimerase chain reaction (RT-PCR). Phenotypic characterization suggests N. friedmannii is non-pathogenic yeast that tolerates UVC (UltraViolet C) radiation and other stresses.Conclusions These features make N. friedmannii suitable for biotechnological applications, adding value to yeast mycosporines as an additive for economically viable, sustainable and environmentally friendly sunscreens. (AU)

• Processo FAPESP: 22/03307-9 - Avaliação da produção de micosporinas e caracterização fisiológica do fungo Papiliotrema laurentii
• Beneficiário: Gabriel Martins da Silva
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 23/08675-9 - Biossíntese de micosporinas por Naganishia friedmannii: uma abordagem biotecnológica para produção de novos bloqueadores solares
• Beneficiário: Gabriel Martins da Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado
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• Beneficiário: Lívia Soman de Medeiros
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 23/11339-0 - Identificação e caracterização de leveduras produtoras de micosporinas: uma abordagem biotecnológica para novos bloqueadores de radiação ultravioleta
• Beneficiário: Renata Castiglioni Pascon
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/04400-9 - O papel da autofagia no crescimento a alta temperatura (37°C) e virulência em Cryptococcus neorformans
• Beneficiário: Marcelo Afonso Vallim
• Modalidade de apoio: Auxílio à Pesquisa - Regular